| In this study, the somatic differentiation and development of Robinia pseudoacacia L. were studied with cotyledon of R. pseudoacacia as explant, and the experimental system of somatic embrygenesis and plantlet regeneration were established. The morphology and histochemistry of somatic embryogenesis were observed by paraffin section. The effect of plant hormones on somatic embryogenesis and physiological and biochemical metabolism of the cell during differentiation and development were investigated. SOD, POD activity and daily growth rate of callus weight were measured. Isozymes in embryonic callus non-embryonic callus and green callus were analyzed by polyacrylamide slab gel electrophoresis and the optimal plantlet regeneration system were established which enriched theory of plant tissue culture. The results were followed:1 The best mediums of callus introduction were: MS+6-BA 1.0 mg·L-1+ 2.4-D 5.0 mg·L-1.2 The best mediums of callus differentiation were followed: the best medium of organ genesis was MS+6-BA 3.0 mg·L-1 + 2.4-D 0.1 mg·L-1 and the best mediums of somatic embryogenesis were MS+6-BA 0.1 mg·L-1 + 2.4-D 1.0 mg·L-1,MS+6-BA 0.1 mg·L-1 + 2.4-D 3.0 mg·L-1.3 The best medium of adventitious bud and root introduction was: MS+ IAA 2.0 mg·L-1 + KT 1.0mg·L-1.4 Mensuration of enzyme activity: browning of callus leaded to decrease of SOD and POD activity, slowness of metabolism and growth situation. And we could checkup growing situation of callus by these two index.5 The polymorphic patterns of peroxidase isozyme and esterase isozyme in different callus types indicated there was close relationship between their composition and activity and differentiation approaches of callus. We could forecast differentiation approaches effectively. |
