The Dynamic Analysis Of Phylohormone On The Time Of Before Drawing Carex Dispalatha To The End Of Divaricate

The saposhnicovia divaricate root is the dry root of the not yet drawing scape plant of theUmbeliferae plant. To using for common cold headache, rheumatic arthralgia, rubella pruritus,tetanus and so on. To follow the period development study it deep hierarchical to utilizing modernscience method. It get full-grown advancement on study chemical composition, pharmacologic action,study the clinical application of praeparatum. But the saposhnicovia divaricate root demand quantumincrease resent a few years, the production and qualitative decrease of the grow wild the saposhnicoviadivaricate root so man-made cultivated area unceasing to expend. Because of soil rarefaction, fertilizerand water adequacy, big yield, had much nutrient substance to accumulate of the saposhnicoviadivaricate root of tame so appearance phenomenon of crudity but draw Carex dispalatha. Beacause ofthe saposhnicovia divaricate root become xylon after drawing Carex dispalatha, protanthesis andfructification so lose the medicine value that for this reason the important factor influence medicinalqualitative of draw Carex dispalatha.of the saposhnicovia divaricate root of tame. The saposhnicoviadivaricate root of tame have drawing Carex dispalatha in second year or third year if misgovern, evenabove 90%. How can control the draw Carex dispalatha of the saposhnicovia divaricate root of tamethat it is a important question for study to the researcher of the saposhnicovia divaricate root nowadays.To determine the contents of IAA (growth hormone),GA (gibberellic acid) and ABA (abscisicacid) before drawing Carex dispalatha to the end of divaricate saposhnikovia root that to clear cut thecontents change of IAA,GA and ABA before drawing Carex dispalathyto the end, for further researchnonage draw Carex dispalatha of divaricate saposhnikovia root to supply according to theory. The testmaterial is the biennial divaricate saposhnikovia root in GAP depot of Jilin Agricultural University thataccredit by professor Zhang-Lianxue. Acquisition time is 2006·5·20—2006·8·20, ten days is a collectionstage, discern collection divaricate saposhnikovia root before drawing Carex dispalatha and afterdrawing Carex dispalatha on the same period. Determine the contents of IAA,GA and ABA beforedrawing Carex dispalatha to the end to untilize HPLC, concrete chromatographic condition: chromatographic column: CentuySILC₁₈ODS reversed-phase column (4.6mm×250mm, 5μm), chromatographic temperature: 35℃, detecton wavelength: 254nm, RF: 0.7mL/min, moving phase: Me:AN:0.7%MA=40:15:45. The contents of IAA and GA in root,caudex,leaf change tendencyalike before drawing Carex dispalatha and after drawing Carex dispalatha of divaricate saposhnikovia,we sampled five times before drawing Carex dispalatha and discoverd that the contents of IAA,GAreveal upgrade tendency slowly, but the contents of IAA,GA decrease sharply after drawing Carexdispalayha, the contents of IAA,GA before drawing Carex dispalayha obviously higher than afterdrawing Carex dispalayha. The contents of IAA in root,caudex,leaf change tendency alike beforedrawing Carex dispalayha to the end, but IAA,GA had comparatively contrast: We sampled five timesbefore drawing Carex dispalatha even if fluctuate small the contents of IAA but retained the same level,the contents of ABA after drawing Carex dispalatha monotonous upgrade sharply and obviously higherthan before Carex dispalatha saposhnikovia root.Through the test to make clear the phytohormone direct influence growth, development and ageingof divaricate saposhnikovia root, The phenomenon of nonage drawing Carex dispalayha of divaricatesaposhnikovia root have graveness relation with endogeny hormone, so we may rudimentextrapolate: the contents of endogeny hormone change straight induce drawingCarexdispalayha of divaricate saposhnikovia root. for further research nonage draw Carexdispalatha of divaricate saposhnikovia root to supply according to theory.