The Research Of Different Gene Expression In Different Tissue Of Mice After Fed With Olaquindox

In recent years, the residues of veterinary drug harm to the worldwide were an issue of great concern,therefore, residue detections become a hot spot. However, the existing detection methods are commonlyexpensive and complicated in operation, also low sensitivity. In this study, we fed mice with Olaquindoxartificially, DDRT-PCR technology for use in mice fed with different organizations around the differentiallyexpressed genes, trying to build the specifical relations between Olaquindox residues and gene expressionfor the establishment of molecular biology detection means laying the foundations.we extracted health Kunming white female mouse liver first, kidney, ovary, Kunming white malemouse testis total RNA, Synthesize cDNA first chain with the 3'end, then amplified PCR with the 5'end ofrandom primer, the amplified products were sequenced by SDS-PAGE and identified the technicalparameters established DDRT-PCR detection technology.We chose Kunming 60 white mice, half male and half female, fed in the ordinary conditions. Femaleand male rats were randomly divided into two groups, a total of four groups. Males and females will bepaired in a group of experimental groups were given 25mg/mL Olaquindox, each 35μL, Another group ofthe mice were given the same dose of water once a day, for five days gavage. Meanwhile we took the liver,kidney, ovary from the experimental group and another group of female mice and testis of male mice. Anddetect the different tissues of differentially expressed genes by those established DDRT-PCR technology to.In the liver was found eight different genes, in renal four were found, in ovarian found one and in the testiswas found two. These two genes after PCR sequencing, amplification the 15 genes we found and comparedwith the mouse genome of GenBank or cDNA, we found three cDNA differentially expressed in renal andnamed F1, F2, F3. According to F1, F2, F3 results of the mensuration sequencing designed differentialprimer, and made a specific RT-PCR amplification to the total RNA of mice kidney in the experimentalgroup and another group. The results showed that these three genes in the experimental group weresignificantly higher expression level than another group.The functional analysis of F1, F2, F3 found that the gene of F1 was homologous with the gene ofprotein phosphatase 1B. Protein phosphatase is a major enzyme to control the protein phosphorylation ofthe human, and plays a key role of regulation in the signal transduction pathway. F2 with the CCCH zincfinger structure contains three cysteine, 8 histidine (zc3h8) and homology of 99%. A zinc finger is usuallyfound in the DNA-binding protein of a structural element, with the structure of the zinc finger protein, mostwith the regulation of gene expression related to the functional protein; F3 Nudix gene with 98% homology, Nudix belonging to two nucleoside phosphate hydrolase family, which has phosphatase activity and existsin the organism substances inducing resistance mutations.We can includ that mice Olaquindox can cause kidney certain gene expression changes from thepreliminary results of the experiment. We can establish the foundation of the molecular biology methods,as well as provide a new scientific means for molecular pharmacology, if these differences in geneexpression between Olaquindox specificity can be determined.