Study On The Abstraction And Antibacterial Activity Of Swine Blood In Vitro

The antibacterial peptide has the broad spectrum antibacterial activity which in the organism to induce and express the micromolecule peptides. These peptides are the organism non-specific immunological response products, and they are the important ingredient in the immunity system of organism.At present the research of swine antibacterial peptides mainly to aim directly at swine intestine and blood in domestic. In domestic they also mainly have progressed the reseach about swine intestine, Although the study on the abstraction and antibacterial activity of swine blood has been few reported, in domestic has not actually seen the report about it. because of among the swine blood the white blood cell excute archfunction in the immunity system, at the same time the neutrophile granulocyte have approximately composes 70% of the quantity in white blood cell, and it is reasonable that abstract swine antibacterial peptide from blood. So the experiment abstract micromolecule peptides in neutrophil of fresh swine blood, according the active characteristic of micromolecule peptides to abstracte and identificate the antibacterial peptides. Meanwhile through grading-up extraction artwork, elevating yield and content of antibacterial peptides.1. The fraction of extractionThe experiment take the micromolecule peptides in neutrophil of fresh swine blood as research object, take the fresh swine blood to anticoagulate and centrifugate, and then processed by supersonic, difussed by acetic acid, latter filtered and laminar analysised by sephadex gel G25,sephadex gel G100, micromolecule peptides below 20KD was separated by ultrafiltration assembly, we collected the composition every step and determined the antibacterial activity of the peptides, gathered the composition that has the antibacterial activity. Using the composition that has the antibacterial activity to carry on Tricine the SDS-PAGE electrophoresis, and we obtained two master strap, molecular mass is about 6KD and 15KD. Through AU-PAGE electrophoresis, we acquired the mastic, and then we used the antibacterial gel overlay assay to determine the antibacterial activity of the mastic, at last we definited the peptides which molecular mass is about 6KD possess antibacterial activity.2. The fraction of bacteriostasis experimentIn view of now the antibacterial peptide we have knew has antibacterial activity in vitro only, and it nearly does not have antibacterial activity in animal in vivo, the experiment has only determined the antibacterial activity of the peptides in vitro. In order to ascertain the antibacterial peptide has broad-spectrum antibacterial activity, the experiment has carried antibacterial activity determination on some different types of strains. The consequence indicate that, the antibacterial peptide obtains by the experiment has antibacterial activity to these strains. The antibacterial activity to E coli. ATCC25922 is the best among them, the minimum inhibitory concentration is 300μg/ml; the antibacterial activity to Li bacillus ATCC54004 is the worst, and the minimum inhibitory concentration is 975μg/ml, we can initially determined the broad-spectrum antibacterial activity through it.