| This experiment makes use of immunohistochemistry, to position the soybean allergic proteins in piglet plica intestinalis and investigate the distributing rule of the soybean allergic proteins in plica intestinalis. Thus, the absorbing rule of allergic macromolecules has been disclosed to some extent.Sixteen Durck×Lndrace×Yorkshire crossbred piglets, selected from two litters, were divided into four groups(A,B,C,D) with 4 replicates(female:male=1:1).During the ages between 22-26d, group A were fed with dairy produce; group B, C, D were fed with milk replacers respectively including purified membrane pilot-plant procedure glycinin, P-Conglycinin, steam-processed P-Conglycinin, taking place of protein in group A by 8 percent.They were totally killed at 26d, tissue samples of duodenum, frontal jejunum, middle jejunum, back jejunum and ileum were taken. Thereafter, paraffin section was made after fixed by paraformaldehyde. Glycinin andβ-conglycinin in each part of intestine tissue were positioned through immunohistochemistry method, and the changing rule of glycinin andβ-conglycinin in intestine tissue has been investigated through making a comparison.The result shows that:1. There can be a certain amount of glycinin in each part of plica intestinalis after glycinin 5d was fed. Tendency of the change of glycinin content in the entire intestine is shaped like figure U. The content seems the highest in ileum. In contrast, it seems the lowest in the middle jejunum. The accumulated optical density of the ileum is extremely higher than the than of the duodenum, frontal jejunum and middle jejunum(P<0.01); besides, it is significantly different from back jejunum(P<0.05). The accumulated optical density of glycinin in duodenum is extremely higher than that in middle jejunum(P<0.01), while there is no significant different from that in frontal jejunum. And also, there is no significant difference between frontal jejunum and middle jejunum in terms of the content of glycinin. The content of glycinin in the back jejunum is higher than in duodenum(P<0.05), and extremely higher than that in frontal jejunum and middle jejunum(P<0.01).2. There is a stable decreasing tendency of steam-processedβ-conglycinin in the entire intestine; while concerning rawβ-conglycinin, the tendency is not evident. However, the accumulated optical density of rawβ-conglycinin in frontal intestine[including duodenum, frontal jejunum and middle jejunum] is significantly higher than that in the back[including back jejunum and ileum](P<0.05). The content of steam-processedβ-conglycinin is basically equivalent as that ofβ-conglycinin in frontal ileum. Besides this, the accumulated optical density of rawβ-conglycinin is higher than steam-processedβ-conglycinin(P<0.2), especially in the middle jejunum, the accumulated optical density of rawβ-conglycinin is significantly higher than steam-processedβ-conglycinin(P<0.05). The accumulated optical density of rawβ-conglycinin in duodenum is the highest, which is significantly higher than the other part of small intestine(P<0.05); the accumulated optical density of rawβ-conglycinin in central jejunum takes a second place, which is significantly higher than that in frontal jejunum, back jejunum and ileum(P<0.05). The accumulated optical density of steam-processedβ-conglycinin in duodenum is also the highest, which is significantly higher than middle jejunum, back jejunum and ileum(P<0.05).3. The accumulated optical density of glycinin, rawβ-conglycinin and steam-processedβ-conglycinin in intestinal villi is significantly higher than that in intestine gland(P<0.01). However, there are little allergic proteins in the peyer's patch.In conclusion, tendency of the change of glycinin content in the piglet's intestine wall is shaped like figure U, that is, it seems an obvious decreasing tendency from duodenum to the central jejunum, while it seems an increasing tendency from the back jejunum to ileum; the rule of allergic distribution ofβ-conglycinin in frontal intestine seems not decreasing but maintaining in a relatively high level, but it seems decreasing in the back intestine; after steam-processing, the decreasing tendency ofβ-conglycinin heated seems fast at first but slow later; the allergic concentration of the glycinin group in each part of intestine is lower than the rawβ-conglycinin group as well as the steam-processedβ-conglycinin group; the allergic content in each part of intestine of the rawβ-conglycinin group is higher than that of the steam-processedβ-conglycinin group(P<0.05); the concentration of allergic protein of the glycinin group, rawβ-conglycinin group and the steam-processedβ-conglycinin group in intestinal villi is significantly higher than that in the recess and the peyer's patch(P<0.01). |
