| β-asarone (cis -1, 2, 4-trimethoxy-5-(1-propenyl)benzene) is a kind ofphenylpropanoid substance which was extracted from Acorus gramineus Soland, and itsinsecticidal activity to stored grain pests was firstly reported by Institute of Urban PestControl, Huazhong Agricultural University. To further vertify the bioactivities andmechanism ofβ-asarone, the toxicity, poisoning symptoms ofβ-asarone against Sitophiluszeamais Motschulsky were investigated under the laboratory conditions. Effects on someimportant physiological and biochemical criteria were tested too. The main results aregiven as follows.Firstly, Biological activity ofβ-asarone to Sitophilus zeamais Motschulsky1.β-asarone had marked posion action effect to Sitophilus zeamai, the LD50 was28.31 ug/cm2 at 96 h by contact treated, and the LC50 was 12.85μL/L by fumigate treated120 h later;2.β-asarone had obvious repellent action to Sitophilus zeamais at 96 h treatment,the mean repellent action were all up to classⅤ;3.β-asarone had quite control effect against Sitophilus zeamais, the LD50 was 0.28g/Kg after 96 h, and it could control the apperance of the new generation, which lower itsgenerate amount. The inhibition rate to progeny was 100.00% when treated at the dosageof 0.5 g/Kg, andβ-asarone had quite defend effect to the wheat, which was as better aschemistry repellent malathion;Secondly, the toxic symptom ofβ-asarone to Sitophilus zeamais MotschulskyThe toxic action response started with excitation, convulsion, paralysis, and death oranabiosis. The characteristic of excitation was crawling exquisitly, then out of line, andknocked down; During convulsion, the foot and antenna twithed or faltered; The insectfaltered a little during paralysis; At last, the foot and antenna ankylosised. The wholetoxic action response mainly represented nerve poison symptom.Thirdly, Effect ofβ-asarone to Sitophilus zeamais Motschulsky biology andphysiology indexes1. The activity of AChE, GSTs, isozyme of esterase and protein content of tissuesuccus in Sitophilus zeamais were repressed trending after treated with the 0.52 g/Kgdosage, whereas the activity of CarE was induced.2. When treated with different dose ofβ-asarone as 0.125, 0.25, 0.5, 1, 2 g/Kg, theactivity of AChE was induced when dealed with the lower dosage, but its activity wouldbe repressed when dealed with the higher dosage; the activity of GSTs was restrained while there were no distinct difference between different dosages; when the dosage≤0.25 g/Kg, the activity of CarE was resrained, while the contrary result was presentedwhen the dosage＞0.5 g/Kg; the contents of protein changed unconspicuously, but itwould be repressed when the dosage＞0.5 g/Kg; the isozyme pattern of esterase wasrestrained more graveness as the hoisting dosage.The whole researches showed thatβ-asarone had many effect ways on Sitophiluszeamais Motschulsky, like contact, fumigate, repellent and population inhabition action.The insecticidal mechanism ofβ-asarone is complicated, which mainly affected theactivities of ACHE, GSTs, CarE, isozyme of esterase and so on. Soβ-asarone was worthexpoiting to become one kind of effective botanical insecticide in the future.
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