Studies On The Preparation And Agents Of Antibacterial Peptide From Musca Domestica

Musca Domestic and Larvae is a good resources of feed resources and medicineresources because of its broad distribution, easy cultivation and lowcost.We started our work in order to find new active antimicrobial peptides. Weestablishing cultivation method for Musca domestica and larvae, finding a newfeeding method for musca domestica with annimal manure. Effects of environmentalfactors on mass reproduction of Musca Domestic Larvae was made in laboratory, theresults showed that the optimum temperature of adult oviposition and larvae growthwas 25℃. The optimum water content of larva diet was 70%. The factors affectinglarva weight were diet, temperature and breeding density in descending order.And then We optimize the antibacterial prptides produced technics. We detect itcan inhibit the resistant-bacterial growth, the MIC and MBC of the anmicrobialpeptide from the Musca domestic larvae was determined, and they can inhibit thegrowth of different species of multi-drug resistant Gram-positive and multi-drugresistant Gram-negative bacteria to different extent. Finally, we determine the effect ofpH and temperature on stability of antibacterial peptide.By the orthogonal design L16(4~5), we determined the prescriptions ofantimicrobial peptide gel: antibacterial peptide (25g); bases (3g); surface-activeagents (0.1g); humectant (20g); water (100g); the optimum pH was 4.5.The inhibitition of antimicrobial peptide spray and gel to resistant bacteria aremeansured. We conclude the spray and gel are potential to clinic use.Finally, the clinical efficacy and the clinical indications of Musca Domesticantimicrobial peptide gel were observed, resulting that the average cure and efficientpercentage of this antibactria peptide gel for severe clinical mastitis were 60% and85%; and the average cure and efficient percentage of 3 days-administrated this gelfor subclinical mastitis were 74% and 88%, and 90% and 94% for 7days-administrated.