Studies On The Tissue Culture Characteristic Of Mature Embryos Of Indica Rice And Establishment Of Plant Regeneration System Of Rice Callus From Mature Embryos Of Indica Hybrid Rice Parents

Mature embryo of Indica Rice has vast application prospects intransgenic studying, because it can be getfreely, but the inductionrate and the differentiation frequency of callus from mature embryoof Indica Rice is very low, the frequency of callus browning is highin subculture period. All this is a barrier to the application ofgenetic engineer to rice breeding. This study use different IndicaRice varieties and Japonica rice varieties as material to analyzethe difference of induction and differentiation of the callus frommature embryos between different varieties in the same tissueculture method. Select 6 Indica hybrid rice parents as material,which possess important application value in rice breeding andoptimize the parameter of tissue culture system to establish theplant regeneration system of the 6 varieties. Select 5 Indica hybridrice parents as material, which posses important application valuein rice breeding, to analyze the influence of copper to the tissueculture of mature embryos with the addition of differentconcentration copper to media at different period of tissue culture.Analyze the difference of agronomic traits between all varietieswith different treatment. All this result from the study willprovide theory basis for applying cell engineer and geneticengineer to improve the varieties. The result are as follows:1. Genotype is a dominant factor in regeneration system ofrice. The culture ability of indica cms line and maintainer line islower than that of conventional indica rice , hybrid Fl generationand restore line. Genotype of the restore line'influence the cultureability of hybrid Fl generation.2. MB medium is suitable for callus induction of mature embryos,Different varities respond differently to different kind andconcentration of hormone,different treatment and differentiationapproach.3. The callus bromning frequency will be reduced with theaddition of copper to the media, the concentration of copper is verydifferent in different period of tissue culture of different varieties.4. The difference of mutation of the same agronomic traits ofdifferent varieties is very great .