Cloning Cry â…  AC Gene And The Study Of Transformation Of Cabbage

Capitate cabbage is an important vegetable,cultivated area has reached to hectare. Capitatecabbage's verminou is very severity that has lost the yield and quality in the production.Lepidoptera insect which include plutellaxylostella and pieris rapae is the main harmful tocapitate cabbage.The usual regulate of cultivate vegetable for resist on the insect has been restricted,becauseof it is hard to find usable resource of the resist on the insect right now.The use of geneticengineer skill which can cultivate new vegetable breed is an important way to the developmentfor agriculture,the skill is not only safe,effective,but also a little of investment,quicklyeffective,specifically kill insect and not pollute environment.Plant genetic engineer providesan effective way.It is said that Bt has been separated from bacteria, Bt's protein hasspecifically poison function,but is safe for highly animal and people.So it is impossible thattransform Bt genetic into capitate cabbage that can attain the source of resist on the insect.This research mainly refered to the cloning of Bt gene,the building of high effectivereproduce for capitate cabbage,the construction of plant expression vector,the obtain ofpositive plants and molecule examine to the transform plants.This lay out the mainly research's result:1 The cloning of Cryâ… AC gene : through the action of restriction,clone the genetic'ssequence which's congenetic reach to 100% that belong to the tape of Cryâ… Ac gene.2 The building of high effective reproduce for the number 3 capitate cabbage:The optimumbuds were induced on induction medium, which was based on MS medium, containing6-BA(4.0mg/L) and NAA(0.2mg/L), When the buds were older, they were inoculated onto therooting medium, which was 1/2MS medium with NAA(0.2mg/L).3 Assurance the Km's filter consistence in the different stage:through Km's filterconsistence in the different stage,we conclude the filter consistence is25mg/L in the budage,but the filter consistence will down to 20 mg/L in the rootage that canattain more positive plants.4 Attainning capitate cabbage of plants through transform:we transform the plantexpression vector including Bt genetic into Agrobacterium tumefaciens,we attain 27 plantsthrough intervene Agrobacterium tumefaciens to the number 3 capitate cabbage, 2 plants havebeen proved that Bt gene had been involved in capitate cabbage's genetic through identificationof PCR and Southern blot.