| Trichinosis is a widespread parasitical zoonosis which harm to human being health butalso economy of graziery and food industry, so it is important in the aspect of diagnosis andtreatment of Trichinosis.Respecting the disservices of trichinosis, it is significant to develop high sensitivity andspecificity diagnostic reagent and high protection vaccine. Using pET-30a as expressionvector, 49ku ES gene was successfully expressed. Production of the McAb againstreconbinant protein of Trichinella nativa is potential serodiagnosis reagents to establishdiagnosis kit for detecting circulating antigen CAg and perfect material for producing geneengineering antibodies.Balb/C mice were immunized by recombinatnt antigen 49ku ES gene of Trichinellanativa. Their spleen lymph cells were fused with SP2/0 cell of mice followed to selecthybridoma cell strain secreting the highest titer McAb and extract and purify ascites. Theactivity of hybridoma cell strain culture medium and ascites were tested with indirectELISA. Class or subclass of immunoglobulin was examined by the kit. Specificity of twoMcAb was tested by Dot-ELISA and Western blot. Cross reaction was tested with adultworm antigen of Schistosoma japonicum, cyst liquid antigen of Cysticercosis cellulosae,polypide antigen of Ascaris suum, polypide antigen of Trichuris suum. Results indicatedthat two strains hybridoma cells secreting McAb against 49ku ES reconbinant protein ofTrichinella nativa were acquired to name D5 and C1. Two strains McAb belong to IgM andκchain. The titer of D5 and C1 strains culture medium and ascites were 1:1280 and1:12800 respectively. The McAb idio-identify 49ku ES antigen at 24 hours. They have nocross reaction with all mentioned antigens. The characterized results demonstrated thatMcAb will be useful in reaserch of Trichinosis and diagnosis method for detectingcirculating antigen.
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