Stevia Extraction Purification And Active Examination Method Research

Along with the lives of the people level enhancement, eats the nutrition now, eats healthily gradually becomes the key point which the consumer cared about. Not only the stevia rebaudiana sugar production can supplement our country sugar the insufficiency, may substitute the saccharine and so on not to favor the human body the chemical synthesis sweetening agent, therefore, the low thermal low fat sweetening agent stevia rebaudiana sugar glucoside also along with it heat, the long-term edible stevia rebaudiana sugar glucoside cannot cause the human to put on weight, is suitable specially the obesity, diabetes, hypertension, the arteriosclerosis, the dental caries sickness patient uses, moreover physics, chemistry performance stable, do not have fermentable. The present paper plants the stevia rebaudiana leaf blade take this laboratory as a raw material, studied and has discussed the stevia rebaudiana sugar glucoside extraction, the purification, the separation, the examination method.The steam boiling soaks the formulation the optimum condition: Take the water as the solvent, the temperature 100℃, the time 6h, the material fluid ratio1:10, soaks raises in the thing the stevia rebaudiana sugar glucoside content is 9.28%.The stevia rebaudiana sugar glucoside depuration has chosen the precipitation effect through the comparison quite good FeSO₄ and Ca(OH)₂ ,Joins the conditionis 80℃; permanent lukewarm water 40 min; static 4h pulls out filters, in through the pocket adsorption resin and the masculine and feminine elements ionic exchange adsorption refining stevia rebaudiana sugar glucoside, after freeze-drying obtains stevia rebaudiana sugar glucoside crystallization powder.The stevia rebaudiana sugar glucoside component separation method mainly has the heavy crystallization law, the chromatographic analysis law. Heavy crystallization law use stevia rebaudiana sugar glucoside each main component the solubility carries on the crystallization differently in the ethyl alcohol solution the main component separation; The chromatographic analysis law is in is loaded with in the silica gel chromatographic analysis column using the stevia rebaudiana sugar glucoside main component to adsorb and the desorption speed differently the stevia rebaudiana sugar glucoside main component separation. The examination stevia rebaudiana sugar glucoside content main method has the weightmeasuring method, the liquid phase chromatography, the thin steel plate chromatographic analysislaw, the ultraviolet spectrophotometric method (anthrone for developer), the ultravioletspectrophotometric method (the DNS reagent for developer), the chemiluminescence law and soon.Uses the silica gel G thin steel plate chromatographic analysis law, launches the system: Normalbutyl alcohol: Ethanoic acid: Can separate extremely clearly three kind of sugars, may determinethe nature of the examination stevia rebaudiana sugar glucoside two kind of main monomercomponentThe ultraviolet spectrophotometric method (anthrone and the ultraviolet spectrophotometricmethod (the DNS reagent for developer) all may determine the stevia rebaudiana sugar glucosidefor developer) the main component, the method is simple, is not high to the test installation request,but the accuracy not high, the error is big.The flowing injection chemiluminescence law determination stevia rebaudiana sugar glucosidewas one new determination method which we studied recently, could determine the steviarebaudiana sugar glucoside accurately the content, compared with other determination steviarebaudiana sugar glucoside determination method, its accuracy, the sensitivity very were all high,this determination method in domestic and foreign still was the blank. Has certain theorysignificance and the practical application value.