Study On Anther Culture Of Processing Tomato (Lycopersicon Esculentum)

The tomato belongs to a subspecies of the cultivated tomato of solanaceae. Tomato flowers cultivated species can make full use of plant germplasm resources,also available to the diversity of characters. Flower cultivation species of these significant advantages, from around the world breeders attached great importance to and actively use, anther culture in recent years become a breeding disease resistance breeding work of a new approach. This paper studies the processing tomato microspore development and the time spent on the relationship between form and anther culture of factors such as gene type, medium, hormones, low-temperature pretreatment, pretreatment mannitol, activated charcoal, and the concentration of sugar and other factors experiments, Development of microspores of the cell morphology, the callus and embryoid bodies of several oxidase activity was analyzed, and embryo and has done a callus-like tissue-specific protein SDS gel electrophoresis experiments.We can see from this paper that:1.Through the tomato microspores with the developmental stage of the flower size, color, anther development between closely related, according to the relationship between them to determine the developmental stage of pollen. To the length of anther development period to determine more reliable, long stimulate the anthers, 2～3mm, pollen spores in the fourth period,when 3～6mm, a single period, and 6～7mm, the two nuclear, three nuclear;2. Genotype is anther culture affect tomato callus pollen and pollen plants of the main internal factors. And extra-cellular material, physiological changes in conditions of small spores development of a material basis, different genotypes anther culture of tomato callus induction was significantly different;3.Medium tomato anther culture is the impact of pollen callus and plant pollen major external conditions. MS medium rate up more than other media;4.In high density conditions, anther culture itself enough to make use of medium conditions, released in a short period of time sufficient volume of factors, the promotion of the development of pollen cells and pollen callus formation. Reason may be "drug-wall", that is anther wall may secrete a substance to promote callus and embryoid bodies are formed, thereby enhancing the rate of callus;5.This study shows that 2,4-D on tomato callus formation and enhance the rate of callus induction has been playing a very important role, From the results we can see that in MS+KT0.5mg/L+2. 4-D 1.0mg/L as induced tomato Callus the optimal .6.Sucrose as sugar callus induction faster, sucrose is not only conducive to anther dedifferentiation callus formation, but also conducive to the growth of callus.7. A specific protein of the embryo formation note the embryoid bodies and more organizations still in the process of formation of protein expression there is a certain difference.