Clone And Expressing Of UDPG In Catharanthus Roseus With Drought-Resistance

In the present experiment, choosing Catharanthus Roseus's root, shoot and leaf as research subject, probe into relationship of compose the cellular of Catharanthus Roseus and the character of UDPG's clone and expression.Overpass the result of solubility-sugar, water potential in plant's root,shoot and leaf in drought stress and the UDPG's rule of expression,open out the composing of cellular in drought stress.It makes for the more diversification rule of cellular.1. Water potential of plants is a major index of drought stress, whose assay could indicate the degree of the soil sample suffered from drought stress, then quantify the lack of water in Catharanthus Roseus.2. The change of sugar concentration is the major emergency mechanism of plant against drought stress, the assay of which could also illuminate how the major apparatus, such as the root, shoot and leaf, respond tO the stress condition.3. As a glycosylation associated protein of secondary metabolite, UDPG could be widely utilized for the synthesis of sugar in the plant's major apparatus; moreover it can regulate the cell wall formation and the change of cell wall in drought condition.The major emergency mechanism of plant is regulation of sugar whose glycosylation donor is UDPG, the study of which may help to figure out the resistant mechanism of Catharanthus Roseus on the molecular level. By measuring the soluble sugar and water potential, in combination with the expression of UDPG gene in root, shoot and leaf, we could confirm that the UDPG gene affects the cell wall formation in drought stress. Furthermore it might offer the possibility to work on the resistance of Catharanthus Roseus against drought in biological technology.Comparing the expression of UDPG gene in drought stress to that in normal condition, we could detect moderate up-regulation of UDPG in treatment of drought, while the increase of sucrose and glucose can be detected remarkably in the root, shoot and leaf part, which could expressly illustrate the main function of UDPG as a glycosylation donor protein.