Sequence Analysis Of ADV And Construction Of Prokaryotic Expression Vector Of VP2 Gene

Based on the sequences of Aleutian disease of mink virus (ADV) published in Genbank, five pairs of primers were used to amplify the genes of MS1,DL1,DL2 and DL3 strains. The genes were amplified by polymerase chain reaction (PCR), subsequently cloned into pMD18-Tvector. The recombinant plasmids were identified by PCR. The sequences of the DNA were obtained and their amino acid sequences were deduced, analyzed and compared The result showed the strains are same as the foreign strains in the special and conserved areas, although the differences from our country. Compared with strains in our country, they are nearly of the same source. We can also infer the areas ADV exists.Two pairs of primers were designed to amplify the VP2 MS1 strain. After harvesting and purifying, VP2 gene fragments were connected into PMD18-Teasy Vector linear, then the gene were cloned into pMD I8-T and transferred into JM109 cells. Positive clones were selected by LB plates with Ampicillin and proliferated, and plasmids were extracted from positive clones. By digestion with enzyme and PCR identification, recombinant expression vectors pGEX-6p-1,pET30a and pMAL-C2.