The Research Of The Application Of Lactobacillus In The Production Of Broiler Chicks And Laying Hens

The experiment is to study the effect of one or compound lactobacillus inchicken appendices on performance. Several strains of lactic acid bacteria wereisolated from intestinal mucous of the chickens and their probiotic roles wereinvestigated by in vitro experiments and in vivo in chickens. The results weredescribed in the following two sections.In the experiment of broiler chicks, the previous selected BLA5 (B5) BLA7(B7) and BLA9 (B9) strains were added into basal diets, to investigate the effectof supplemental Lactobacillus spp. on performance and cecal microflora in broilerchicks, one day old of six hundred male chicks were divided into the control(noLactobacillus),BLA5, BLA7, BLA9, B5+B7, B5+B9, B7+B9 and B5+B7+B9, isolate in Expt1. Microbial number was confirmed to 10~7 cfu/g diet. Diets contained CP 22.20%andME 3,100, 3,100kal/kg for starting and finishing period, respectively.Body weight increased significantly in supplemental Lactobacillus treatmentsfrom 2 weeks of age (P＜0.05) compared to that of control. Feed intake and feedconversion were not significantly different, whereas the feed conversion improvedslightly in supplemental Lactobacillus treatments.The number of cecal Lactobacillus spp. tended to increase in supplementalLactobacillus treatments at 1 week of age, but was not statistically different.The number of total yeast in cecum was found no difference between Lactobacillustreatments and control.As the result, Lactobacilhs feeding increased weight gain and the number ofintestinal Lactobacillus of broiler chicks irrespective of strains.In this experiment of iaying hens, a feeding trial was carried out to influenceof dietary supplemental Lactobacillus on productivity, egg quality and intestinalmicroflora in 320 21wk-old laying hens for 12 week. The previous selected LLA7(LA).LLA9(LC) and LLA11(LV) strains were added into basal diets with none, LA,LC, LV, LA+LC, LA+LV, LC+LV and LC+LC+LV. Dietary microbes was confirmed to 10~7 cfu/gdiet.Egg production tended to increase as dietary supplemental Lactobacillus increased but was not significantly different. Average egg weight weresignificantly higher in adding Lactobacillus (P＜0.05 ) than control. Theincrement of egg weight in Lactobacillus group were great higher in latter feedingperiod of laying hens. Daily egg mass also increased in adding Lactobacillusapproximately 1.1 to 2.3g/hen compared to that of control, but was notsignificantly different. Feed intake and feed conversion were found no difference.Egg shell breaking strength and thickness were not difference regardless ofLactobacillus supplementation or laying period. Haugh unit improved with addingLactobacillus. Cecal Lactobacillus spp. also increased significantly with addingLactobacillus(P＜0.05). However, the number of E. coli were not depressed bydietary supplemental Lactobacillus.The result of this experiment suggested that dietary supplementalLactobacillus could improve the egg production, egg weight and egg mass.