Genetic Transformation Of Isopentenyl Transferase Gene To Thinopyrum Intermedium And Regeneration Of Transgenic Plants

Plant regeneration is very important in transgenic Thinopyrum intermedium.Explants were directly cultured on modificated MS solid medium containing 2.4-D5mg/L and BA 0.1mg/L to induce calli and then transferred to MS mediumcontaining ZT 0.5mg/L, NAAlmg/L and COCl₂ 5mg/L for callus differentiation and1/2 MS medium for rooting. It was found that the callus induction frequency was79.2% and plant regeneration frequency was 30.2%. Isopentenyl transferase (ipt)gene was transformed into Thinopyrum. Intermedium by Biolistics bombardment andLaser microbeam puncture technique upon selection with kanamycin 300mg/L, thekanamycin resistant calli were obtained and some transformed plants were recoveredin vitro. The transgenic plantlets of Th. Intermedium were identified by PCR analysisof digested genomic DNA and southern blot.