Influence Of Pesticide Intimidation On The Isoenzyme In Body Of Wolf Spider Pardosa Pseudoannulata

It was first reported that the intimidation of insecticide influencedPOD and EST isoenzyme in the body of wolf spiders (Pardosapseudoannulata) in the farmland in the long time. And improved theexperiment method on benzidine dyeing of POD enzyme when the roomtemperature was below 10℃. The effect of the dyeing is improve in60℃water bath. It created conditions for the similar research work infuture. These results were discussed as follows.1. Using the vertical slab polyacrylamide gel electrophoresistechnology to four species of spiders in the rice fields: The POD and ESTisoenzymes of Pardosapseudoannulata, Pardosa laura Karsch, Trochosawuchangensis and Dolomedes pallitarsis were comparatively researchedin this thesis. The results showed that the POD and EST isoenzyme bandsof four species of spiders were trenchant and there were obviousdifferences in different species(P＜0.05), and it indicated that POD andEST isoenzymes of the same species had good stability under the sameenvironment.2. Giving emphasis to analyse the influence of long-term insecticideforce on POD and EST isoenzyme in the body of the wolf spidersPardosa pseudoannulata in the rice fields: with the electrophoresisseparation technology, to compare the POD and EST isoenzymes bandsof Pardosa pseudoannulatas that were collected from applyinginsecticide rice fields in Leifeng Town and no-applying insecticide ricefields Tianding Town, it was found that there were obvious adaptabilitychanges of POD and EST enzyme in the body of the wolf spider under intimidation of insecticide, and the types of enzyme bands of Pardosapseudoannulata that collected from applying insecticide rice fields weremore than those from no-applying insecticide rice fields. The PODenzyme had five kinds of enzyme bands with different Rf. Four enzymebands Rf=269,Rf=33,Rf=122 and Rf=170 among them didn't appearin the no-applying insecticide population, and more modalities of theapplying insecticide population were strengthened obviously. The Rfenzyme bands of EST1 ( Rf=30),EST4 (Rf=215～232),EST6(Rf=332～345) and EST7 (Rf=336～375) were seen only in applyinginsecticide population of Leifeng Town among eight kinds of enzymebands of EST enzyme with different Rf, not seen in no-applyinginsecticide population of Tianding Town, and concentration of enzymebands of species that collected from applying insecticide rice fields inLeifeng Town were higher than those from no-applying insecticide ricefields in Tianding Town.At the same time, in order to verify the active changes of PODisoenzyme, in the condition of the longest absorbability wavelength385nm, the thesis applied the spectro photometry to test that theabsorbency A of the POD enzyme of applying insecticide population was0.86 in Leifeng Town, but that of no-applying insecticide population was0.58 in Tingding Town. The result indicated that the absorbency ofapplying insecticide species was far more than that of non-applyinginsecticide population, it was proved that the POD enzyme activity ofPardosa pseudoannulata was enlarged under the long intimidation ofinsecticide force.Why did the above differences appear in the same nature populationof the same geography conditions? The author considered, it was the main factor that chemistry pesticide forced the wolf spider in the ricefields in the long time. and Pardosa pseudoannulata had producedprobably adaptability mutation and heredity differentiation for the forceof pesticide.3. It was studied that the short-term intimidation of insecticide(itsconcentration is 0.0025 times of concentration of the original fluid)influenced POD and EST isoenzyme in the body of young Pardosapseudoannulata in the laboratory. It was found that the types of PODenzyme bands of Pardosa pseudoannulata wasn't changed under theshort-term intimidation of insecticide force in the laboratory and it'sconcentration is largened, the types and concentration of EST enzymebands of Pardosa pseudoannulata was largened in the short-termintimidation of insecticide in the laboratory, The author considered, It wasthe short-term intimidation of insecticide in the laboratory that hadstimulated POD and EST isoenzyme in the body of young Pardosapseudoannulata to produce.