Organelles Proteomic Studies Of Cytoplasmic Male Sterility And Maintainer Lines In Triticum Aestivum And Zea Mays

Comparative studies of chloroplast proteome on different developmental stage (seedling, tillering, shooting, booting stage) of leaves have been made in isoplamic allonuclear male-sterile lines Nongda 3237A, Xiaoyan No.6A and their maintainer lines by 2D-PAGE. The results indicated that no obvious differences were found in chloroplast proteome between Nongda 3237A, Nongda 3237B, Xiaoyan No.6A, Xiaoyan No.6B at different developmental stages of leaves. Differences were found just at booting stage in Xiaoyan No.6A and its maintainer lines. Obvious differences, however, were observed in chloroplast proteome between isoplasmic allonuclear male-sterile lines Nongda 3237A and Xiaoyan No.6A. For instance, 2 protein spots (pI5.4/34kDa, pI5.4/32kDa) at seedling stage, 6 protein spots (pI5.4/80kDa, pI5.4/65kDa, pI5.4/60kDa, pI5.4/48kDa, pI5.4/40kDa, pI5.4/35kDa) at booting stage were present in Xiaoyan No.6A and absent in Nongda 3237A. pI6.3/18kDa was present at seedling stage in Nongda 3237A and absent in Xiaoyan No.6A. pI6.8/28kDa protein spot revealed the developmental changes. It was present in leaves at seedling, tillering, shooting stages and absent at flowering stage in Nongda 3237A. No development changes of the protein spot were observed in Xiaoyan No.6A.These experiment results demonstrated that it was possible chloroplast proteome wasn't relative to the cytoplasmic male-sterile characteristics. But nuclear background in male-sterile lines can obviously affect chloroplast protein composition. Distant relative on nuclear-cytoplasmic has large differences on chloroplast proteome than close relative on nuclear-cytoplasmic in CMS.The mitochondria proteome of T-cytoplasmic male sterility (T-CMS) and its maintainer of leaves (seedling, shooting, booting stage), mesocotyls, roots and anther (pollen mother cells meiophase, pollen microspore single-double nuclei stage) were analyzed. PDQuest analysis showed that about 350 protein spots in seedling leaves, 150-200 protein spots in mesocotyls and roots, 150 spots in anther were identified. The results indicated that obvious differences were found in mesocotyls, roots and anther (pollen microspore single-double nuclei stage). Mitochondrial proteome of different stages in leaves showed significant changes, but no difference between T-CMS and its maintainer. In pollen microspore single-double nuclei stage, there are great differences between T-CMS and its maintainer, which is consistent with the pollen sterility. Using MALDI-TOF MS, relegation identification of different proteins between T-CMS and its maintainer were performed. R40c1 protein (in mesocotyls), mature anther-specific protein, DNA-directed RNA polymerase 23kD submit, ribosomal protein L5 and putative polyprotein were only observed in T-CMS. Glutathione S-transferase and signal transduction inhibitor were just found in maintainer.