| In view of decreesing of photosynthesis productivity at present, which were resultedfrom high light, especially high temperature and high light during production, thisexperiment as tried the material take the eggplant named 'HEIBEI', under the differenttemperature, light intensity, light quality, as well as dark adaption and the adaxial andabaxial surface of vivo or vitro leaves, has measured the chlorophyll fluorescence dynamiccueves and parameters of leaves, including Fo, Fro, Fv/Fm as well as JIP-test parameters,studied photoinhibition effects of high light and monocolour light on eggplant leaves.Results shows the leaves take place photoinhibition not only in high light, but also in lowlight and single light, which degrees of photoinhibition are different; meanwhile, degreesof photoinhibition will aggravate along with increasing of treatment time.In the 3000μmolm-2s-1 high light treatment time to the vitro eggplant leaves,chlorophyll fluorescence dynamic cueves (O,J,I,P) changes as the bigger extent:fluorescence intensity at the O-step increase as little extent along with increasing oftreatment time (from 0 min to 25 min as increasing by 5 min); meanwhile, three steps (J, Iand P) decrease gradually along with increasing of treatment time, and indeedly disappeargradually. In the treatment of 25 min, chlorophyll fluorescence dynamic curve became theshape of beeline almostly, which characteristic steps (J, I and P) disappear. Quenching ofchlorophyll fluorescence aggravate along with increasing of treatment time: the maxicalphotochemical efficiency Fv/Fm decrease gradually; dissipated heat energy increasegradually; photoinhibition aggravate gradually.In high light combining treatment of time (or light intensity) and leaf temperature, theeffects of normal temperature 25℃and high light on chlorophyll fluorescence dynamiccurves are weak, concomitantly increasing of fluorescence intensity at O-step as littleextent and decreasing of three steps (J, I and P) as little extent, which take place gentlephotoinhibition; low temperature 10℃and high ligh aggravate photoinhibition, three steps(J, I and P) decrease obviously; high temperature 40℃and high ligh aggravate furtherphotoinhibition, three steps (J, I and P) decrease further. Through single light by Tungsten Halogen Lamps treatment, results show that 530 nmGL which has lesser photo flux density affect PSⅡhighly at the decreasing of Fv/Fm andRC/CSo, the increasing of Fvi/Fv, as well as the decreasing of photosynthesis driving forceDFCSo and the components; meanwhile, results show effects different wavelength lightfrom 530nm to extending light wavelength to two sides (red light and blue light) on PSⅡbecome low graduly, which photoinhibition become low graduly.Through single light by LED treatment, results show that effects of 470 nm blue lighton chlorophyll fluorescence dynamic curves are the bigger, next is 530 nm green light,again is white light as blending light quality, and final is 620 nm red light; throughmeasuring of light absorption by global integraph, results show leaves has the most lightabsorption to 620 nm red light, next is white light as blending light quality, again is 470 nmblue light, and final is 530 nm green light.Through measuring chlorophyll fluorescence dynamic curves of high light treatmentto light adaption and dark adaption, decreasing of fluorescence intensity at P-step, Fv/Fmand RC/CSo, as well as increasing of VJ at J-step, indicate that leaves in dark adaption takeplace photoinhibition more easy than that in light adaption to result decreasing ofphotosynthesis activeness.Through measuring chlorophyll fluorescence dynamic curves of high light treatmentto the adaxial and abaxial surface of leaves, results show characteristic steps (J, I and P) inthe abaxial surface of leaves are under characteristic steps (J, I and P) in the adaxial surfaceof leaves no matter what in control or treatment. |
