Transfer Of Bivalent Insect Genes Into Soybean Mediated By Agrobacterium Tumefaciens

Soybeans were injured seriously by pests during the period of duration, which resulted in great loss of production of soybean. The widely use of chemical insecticide not only enhanced pesticide resistance, but also destroyed the useful insects and other ecological flora. Also, it polluted environment seriously, and increased the cost price of produce, and it broke the balance of ecology. Because the produce of breeding was too long, and the genetic resource were limited, and the mechanism of pest resistance was unknown, as well as the development of new pests resulted in the instability of resistance, the routine method of breeding and planting technology were limited in some extent, which prove effects were little. As a result, it has been paid close attention that using plant genetic engineering technology to enhance the pest resistance.The pGBI121S 4ABC carried two insecticidal genes,CpTI and GFM CryIA B.t, were successfully transferred into 2 soybean (Glycine Max) cultivars,"Jilin 20"and"Jilin 27"via Agrobacterium tumefaciens mediated transformation. Immature soybean cotyledons were used as recipient. After co-cultivation of Somatic embryogenic callic induced from the immature soybean cotyledon as recipient materials with A. tumefaciens on screening medium, somatic embryos and regenerate fertile transgenic plants were obtained. Transgenic soybean plants harboring two insecticidal genes were confirmed by PCR and GUS testing.