Study On Tissue Culture Of Different Cultivar Groups Of Osmanthus Fragrans

The stem apical of different cultivar groups of Osmanthus fragrans were used as explants in this study to explore the affection of the different basic mediums and the combination of the different plant growth regulators to the cultivar groups' germination of stem apical,proliferation and rooting ability.Meanwhile the test-tube plantlets' leaves of 'jiingui'and 'dangui' were taken as explants to show induction and proliferation of callus.Single factor random block,orthogonal design and uniform design were adopted to arrange the experiment.The major results were as following:1.The concentration of disinfectant mercuric chloride for stem apical of 'jingui', 'yingui' and 'dangui' was 0.1%.The suitable range of disinfection time was 3～7min, and the best time was 5 minutes.The best explants were stem apical which was easier to be disinfected and had higher survival ratio than stem.2.The optimal mediums of stem apical primary culture for each cultivar groups were as following individually.For 'jingui'was B5+6-BA2mg/L+NAA0.12mg/L,and with germination ratio 86.1%;For 'yingui' was B5+6-BA2mg/L+NAA0.02mg/L,and with germination ratio 85%;For 'dangui' was B5 or LMC+6-BA7mg/L,and with average differentiation 1.73.The predominance difference of ratio of the germination among cultivar groups were 'jingui'＞'yingui'＞'dangui'＞'sijigui'.There was a great similarity between the performance of 'jingui' and 'yingui' in the stem apical primary culture;Much callus appeared in the stem apical primary culture of 'dangui' which seriously prevented the germination ratio.The stem apical primary culture of 'sijigui'had almost failed.3.There were different optimal mediums for proliferation culture among cultivar groups of Osmanthus fragrans.The optimal medium for each cultivar group were as following.For 'jingui' was B5+6-BA5mg/L+NAA0～0.05mg/L,with the proliferation times 2.5;For 'yingui' was B5+6-BA7mg/L+NAA0～0.05mg/L,with the proliferation times 2.2,For 'dangui' was B5+6-BA8mg/L,with the proliferation times 2.8.High concentrations of NAA could prevent proliferation of the different cultivar groups of Osmanthus fragrans.4.The optimal medium for rooting of 'dangui' was 1/2B5+6-BA0.05mg/L +NAA1.5mg/L or 1/2B5+6-BA0.1mg/L+NAA1.5mg/L.That combinations could get a better rooting quality,such as the ratio of rooting rate was high to 100%,the average root length was 0.5cm,the average rooting number was 2.38. IBA was not an effective hormone for 'dangui'rooting5.As to nursling of plantlets,the medium with 1/2 vermiculite and 1/2 grass char was the best material,which led to the survival rate of plantlet up to 80%.6.The optimal medium for callus induction of 'dangui' leaf was B5+6-BA0.6mg/L+2,4-D0.6mg/L+sugar30g/L+agar5g/L.The optimal medium for callus induction of 'jiingui' was B5+6-BA1.0mg/L+2,4-D0.6mg/L+sugar30g/L+agar 7g/L.In addition,the callus of leaves of test-tube plantlets could only be induced in B5 basic medium with low concentration of 6-BA.The callus of 'dangui' and 'jingui' could proliferate well in the optimal medium for callus induction.