Effect Of Schiff's Base Of Lysine On The Flow Of Nutrients To The Small Intestine And Rumen Digestion And Metabolism Of Sheep

The in vitro antibacterial activity of the Schiff's base of lysine, the effects of supplementing lysine and Schiff's base of lysine were studied on flow of protein, liquid free amino acids to the small intestine and rumen digestion and metabolism.The Schiff's base of lysine was synthesized by lysine and salicylaldehyde. The minimum inhibiting concentration against E.coli,D. aureds and Salmonella were determined by concentration dilution method. Bactericidal curve was made by three times MIC of Schiff bases of lysine. The results showed that the minimum inhibiting concentration were 786.4, 1228.8 and 983μg/mL, respectively. Antibacterial activity of three times MIC tended to be similar after 4h.Four male China merino(Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design trial to study the effect of supplemental Schiff's base of lysine 3.42g/d, 6.84g/d(the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g , 5.12g/d(the content of lysine is 2g and 4g respectively) on the flow of protein and liquid free amino acids to the small intestine. The results indicated that 6.84g/sheep/d Schiff's base of lysine equal to 4g free lysine increased flow of Lys, Glu and Tyr to the small intestine by 25.17%, 38.35% and 33.88%(P<0.05) respectively. Flows of other free amino acids to the small intestine were not affected by supplementing with Schiff's base of lysine. Supplementing Schiff's base of lysine did not affect crude protein intake, microbial protein synthesis and flow of rumen undegradable protein to the small intestine.Four male China merino (Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design to study the effect of supplementing Schiff's base of lysine 3.42g/d and 6.84g/d (the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g and 5.12g/d(the content of lysine is 2g and 4g respectively) on rumen digestion and metabolism. The results showed that Schiff's base of lysine with 4g lysine decreased the concentration of the total volatile fatty acids and acetic acid by 7.24% and 9.44% (P<0.05), respectively. The average concentration of butyric acid increased 10.64% (P<0.05) with supplementing Schiff's base of lysine from 3.42g/sheep/d to 6.84g/sheep/d. The average dry matter intake , pH, concentration of NH3-N and rumen protozoa in the rumen were 1374.21～1419.01g/d, 6.28～6.40, 16.44 mg/100ml～18.31 mg/100ml and 86.31×10~4～89.91×10~4 respectively, with no significant difference among groups. Rumen apparent digestibility of dry matter, organic matter, cellulose and hemicellulose were not affected by supplementing Schiff's base of lysine.It was concluded that 6.84g of Schiff's base of lysine increased the flow of Lys, Glu and Tyr to the small intestine, and reduced concentrations of acetic acid and total volatile fatty acids in rumen.