Cloning And Expression In E.coli Of Porcine CD58 Gene

CD58, also was called lymphocyte function-associated antigen 3, is a surface glycoprotein expressed on many cell types, binds to the T lymphocyte surface glycoprotein CD2. This interaction is important in mediating T lymphocytes with target cells and antigen-presenting cells, and the T lymphocyte rosetting with erythrocytes. It plays an important role in mammal immune system.In this study, a pair of primers was designed and used to clone porcine CD58 gene by RT-PCR method. The results showed that a 800 bp fragment of porcine CD58 cDNA with an ORF of 735bp was obtained. Then, this cDNA was ligated into pGEX-4T-1 and transformed to E.coli BL21 cells. The recombinant transformant was induced with IPTG and the expressed product could be recognised by anti-CD58 serum. The prediction of the protein structure indicated that the porcine, sheep and human CD58 have high similarity, especially in V region which was the molecular foundation of xeno-adhesion between lymphocyte and RBC. This research will help us to develop new adjuvant and/or immune-regulated drugs, and laid a solid foundation for further study of structure of CD58 molecule and the mechanism of CD2-CD58 complex triggering immune system.