| The drought-resistance spring wheat (Triticum aestivum L.) are the mainly cultivated typies in northwest of our country. Drought and shortage of water are both hinder the production steadily increasing. However, with the water resource decreasing day by day, the traditional irrigation farming is unfit. The only way to solve the problem is to discover germplasm resources in our province, assemble different drought-resistance genes in order to get the duration genes. The RAPD maker needs not design primer and the operation was easy, so it is farily fit to analysis large sample, which has been widespread appled to many different cultivars.Through the paper collected 47 different drought-resistance sping wheat, including the predominant cultivars in northwest , the new breeded cultivar and triticale,studied on them by RAPD and the index of different drought-resistance in cultivars'seedling stage,aimed to analysis the genomic diversitfication in the predominant cultivars in our province,and searched the index of drought-resistance.Thus we can get information in order to breeding new cultivars.The main experimental results are as followings:(1) A tree diagram of drought-resistance was constructed by studing the seedling stage index of different drought-resistance cultivars.All the materials were taxonomied into 4 typies: the frist kind including 6-90,7015,21351,Lijian8,they were spring wheat in humid land;the second kind had poor drought-resistance including Dingfeng889,9053,1513,Xiaohongmai,Xiaojian5,Gaoyuan671,Longfu3 and 15-2-106;the third kind had strong drought-resistance including Dingxi38,Dingxi37,1533 and Xihan1;the rest 31 cultivars were classed to one kind,their drought-resistance were middling.(2) The optional reaction RAPD system was setup for the assay and usagein wheat by testing and comparing some essential factors. The reaction program consisted of 4 cycles of prede-naturation at 94℃for 3min , an nealing at 37℃for 1min ,and extension at 72℃for 2 min, followed by 40cycles of denaturation at 94℃for 1 min, annealing at 37℃for 1 min , and extension at 72℃for 2 min. The final cycle had a 10 min extension at 72℃.The PCR mixtures of 25μL contained 25ng template DNA, 0.2 mmol/L of dNTP, 2.5mmol/L, Mg2+, 10pmol primer and 0.75U Taqpolymerase.(3) Amplified the genomic DNA of 47 wheat by RAPD, the number of DNA bands was between 3～13, the molecular weight was concentrated between 250bp～2500bp. 134 DNA bands were amplified in total, 122 of that were polymorphic(91.4%), which showed that there were quite high genetic diversity among different genetic wheat. (4)A tree diagram was constructed by using NTsys2.0 method. All the materials were taxonomied into 6 typies: among them, 02-7570 its inheritance compared with other breeds relationship was far away, which could be divided alone; the genetic relationship near it were SW1026,Xiaojian8,92067 and the strong drought-raesistance Longchun 20,etc. 21 cultivars were devided into on kind; Z18,9053,Z32,9054-15 were divided into one kind; devidd the breeded new cultivar Xihan 1and 29,Z5,91050,Xiaopin20-1528 into one kind; Z36,93054-3,Z45,94474,54,Dingfeng889 had near relationship; the rest twelve cultivars were classed to one kind.
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