Several Kind Of Apples Stocks Tissue Culture Technology Research

The experiment was carried on in 2004-2006 year in the Shandong Longkou and the Shandong Institute of Forestry Science courtyard, Research was carry on how to reduce the cost in view of the low reproduces coefficient, low rootage and transplanting, not formed the scale to produce, the perfect group cultivates and the seedlings technical system to the factorization to carry on to the optimized so the technical system effectively reduce the single cost.Meanwhile the research of Malus halliana koehne was carry on in order to enhance the flowers quality and the plants breeding speed, guarante the plant hereditary uniformity, achieve the anniversary continuous production and provides the market supply. The result as follows:1.Take apple stock M9 and M26 as material, established the aseptic system the best generation culture:M9:MS ( NH4NO30.8g/l,KNO32.28g/l )+IBA0.3mg/l+6-BA0.6mg/l+Argar8g/l,differentiation coefficient can up to 4.41 ; M26: MS+ IBA0.5g/l+6-BA1.0mg/l+ Argar8g/l, differentiation coefficient can up to 4.59.The best rooting medium : M9:1/2+IBA0.3mg/l(K2SO41188mg/l)rooting coefficient up to 84 % ;M26 : 1/2WPM+IBA0.3mg/l , rooting coefficient 98.7 % .The best domestication humus: M9:Perlite:Peanut shell:Peat=1:1:1 , the achievement percent is 85%。M26:Perlite:Peanut shell:Peat =1:1:1,the achievement percent exceed 95%。Contrast with the cost it can be fall from 1.19 Yuan to 0.60 Yuan if the anniversary production and the simplification group system was carryed out.2. Conventional tissue culture methods and orthogonal design of Malus halliana koehne was carry on explants disinfection, rapid propagation of culture, leaf callus induction and regeneration process to take root, and other research. The explants best disinfectant time : 75% alcohol 30s+0.1% mercuric chloride 10min.The best medium for the proliferation : MS+6-BA0.7mg/L+NAA0.3mg/L, multiplication coefficient can up to 7; Orthogonal design results showed the size of hormone on the leaves callus induction: 2, 4-D>6-BA>NAA. The best reproduce medium was 6-BA6.0 mg/L +NAA0.5 the regeneration coefficient can up to 91.8% ,1/2MS+IBA0.3+sucrose 20g/L was the best rooting medium...