| The DNA molecular marker technology, such as PCR-SSCP, PCR-Sequencing, PCR-RFLP, were applied to analyze the genetic variation of H-FABP gene and DGAT1 gene in ShaanBei White-Cashmere goat population, aged one year. The association between genetic markers and the performance traits, including some growth and carcass traits, were analyzed by using general linear model(GLM);the resulit were as followed:1. The thickness of backfat plus skin and loin area in ShaanBei white-Cashmere goat were measured by using the B-mode ultrasound scanner.2. The size of some exonⅡ,exonⅢ,exonⅣand some 3'-UTR was 132 bp,102 bp ,54 bp and 75bp respectively while the size of intronⅡand intronⅢwas 1 886 bp and 1 489 bp respectively. The junction sequence of exons and introns of its H-FABP gene was in accordance with the law of gene composition. In addition, The homology rates of some gDNA sequences of the H-FABP gene between the ShaanBei White-Cashmere goat and cattle, pig were 93%, 89% respectively.The nucleotide sequence of the coding region among different species, including ordinary goat, sheep,cattle,pig, were quite conservative while there were some difference between them..For example, there were a transition (C→T) in its fifteenth position and an inversion beteen its 176th position and 177th position by comparing some coding region sequence beteen the ShaanBei White-Cashmere goat and ordinary goat.3. The analysis results of the SNPs of H-FABP gene showed: These locuses of EXONⅡLocus and INTRONⅢA Locus were not at Hardy-Weinberg equilibration(p<0.01)in the ShaanBei White-Cashmere Goat.Their polymorphic information content (PIC) were 0.1052, 0.3925 ,which meaned low diversity and moderate diversity respectively. A mutation, G→C, was discovered in INTRONⅢB Locus by using PCR-Sequencing method, but didn't cause amino acid sequence changed.The enzyme that could identify and cut the SNP mutable point wasn't discovered temporarily. No polymorphism was displayed in INTRONⅡLocus which was showed with 8 bands and 3 bands respectively after being cutted by HaeⅢand HinfI enzyme by PCR-RFLP method. They were appeared as one allele and one genotype.The results of this research showed that there was one mutation in the 22nd site of the exonⅡ(G→C)which caused the glycine( G) into arginine( R).There was significant difference between the genotype of AB and AA as well as between the genotype of AB and BB while there was a significant difference between the genotype of AA and BB for the back-fat thickness. There was significant difference between the genotype of AB and BB for the heart girth and Circumference of cannon as well as between the genotype of AA and BB for the loin eye area. In addition, there was no significant difference for other traits. The trend of"AA>AB>BB"was noted for six traits of birth weight, loin eye area and back-fat thickness et al, the trend of"AB>AA>BB"was observed for three traits of wither height, heart girth and circumference of cannon. The results of this research showed that there was two mutations(G→A and T→C ) in INTRONⅢA Locus ,There was significant difference between the genotype of AA and CC for the yearling weight(p<0.05)while there was significant difference between the genotype of AA and BB for the body length(p<0.05)in the ShaanBei White-Cashmere goat. There was significant difference between the genotype of AA and BB as well as between the genotype of AA and CC for the loin eye area(p<0.05). The trend of"AA>BB>CC"was noted for four traits of weaning weight, yearling weight, heart girth and loin eye area while the trend of"AA>CC>BB"was noted for the birth weight. what's more, The trend of"BB>CC>AA"was noted for three traits of wither height , body length, circumference of cannon while the trend of"CC>BB>AA"was noted for the back-fat thickness .4. PCR-SSCP technique was used to analyze the polymorphisms of the DGAT1 gene. The analysis results showed: there was a pair of alleles and the population was not at Hardy-Weinberg equilibrium; Their polymorphic information content (PIC) were 0.1029 which mean low diversity.The results of this research showed that there was one mutation(G→A)in the eighth site of the exon14 in the DGAT1 gene which caused the glycine(G) into glutamic acid(E).There was significant difference between the genotype of AA and BB as well as between the genotype of AA and BB for the four traits of weaning weight, yearling weight, heart girth, circumference of cannon(p<0.01),but not between the genotype of AA and BB for them.The trend of"BB>AA>AB"was noted for three traits of birth weight, yearling weight,heart girth while the trend of"BB>AB>AA"was observed for three traits of weaning weight, wither height, circumference of cannon. Moreover, the trend of"AB>BB>AA"was noted for body length and the trend of"AB>AA >BB"was noted for back-fat thickness while the trend of"AA >BB >AB"was noted for loin eye area . |
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