Analysis Of Genetic Diversity Germplasm Using SSR And Construction Of The Core Collection In Vitis Amurensis

Vitis amurensis originate from Northeast in China.It has the most cold hardiness in Vitis species, the branches and tendrils could resist-40℃, and the roots could resist-14～-16℃, and it has strong resistance to powdery mildew, white rot, ripe rot, anthracnose, downy mildew, and without the foxy flavor that limits the use of V.labrusca in breeding programs. They are used as valuable resources of cold hardiness and disease-resistance in grape breeding. SSR was used to research the genetic diversity of 124 V. amurensis germplasms, and constructed the core collection of V. amurensis preliminary.Selected nine SSR locis of eight V. amurensis varieties were exhibited higer polymorphism, and determanated length of amplification segment. Eight V. amurensis varieties may be well distinguished by using the nine locis26 pairs of SSR molecular markers were used to analyze the genetic diversity of 124 V. amurensis germplasms, and the preliminary results indicated the total genotype numbers from 124 V. amurensis germplasms were 210. The average allelic gene number(Na) were 3.6923, The effective allelic number(Ne) were 2.5779, and Shannon Index of diversity(I) were 1.0195, the average expected heterozygousity(Nei's) was 0.5762. The genetic diversity of wild germplasms is higher than cultivar; the genetic diversity of Heilongjiang is the highest, Jilin is the next, and Liaoning is the lowest in the wild germplasms.The genetic similarity of V. amurensis germplasms coefficient(J) was 0.224～0.972, the average genetic similarity coefficient was 0.588. The clustering dendrogram was constructed by UPGMA method. The 124 Vitis amurensis germplasms was divided by 26 pairs of SSR molecular markers. The V. amurensis germplasms were divided into 6 major groups while similarity coefficient is 0.39. Majority germplasms clustering was according with the distribution of V. amurensis.According to the cluster result of SSR, we employed three methods to construct core collection of V. amurensis germplasm, camparing the genotype numbers, the average allelic gene number, the effective allelic number, the Shannon Index of diversity, and the average expected heterozygousity, the results showed: employed the method of sampling progressively by clustering to construct core collection, which had the better representativeness. In consideration of function of V. amurensis variety and staminiferous plant V. amurensis in production and scientific research, V. amurensis variety and staminiferous plant V. amurensis were also merged into core collection. The core collection involved in 41 germplasms(33.1%).15 wild germplasms from Heilongjiang(36.6%), 10 wild germplasms from Jilin(24.4%), 8 wild germplasm from Liaoning(19.5%), 8 cultivar germplasms(19.5%); 27 female flower germplasms(65.8%), 7 hermaphroditic flower germplasms(17.1%), 7 staminiferous plant germplasms(17.1%). The results showed that the core collection hold 33.1% samples of initial collection, the reserved rate of observed number of alleles, effective number, of alleles, Nei's gene diversity and Shannon's information index were reached 86.7%, 97.9%, 100.0%, 99%and 99.8% respectively. The above results showed: the constructed core collection evaluated representatively V. amurensis germplasm.