Selecting Of Excellent Strains Of Mutation Crossbreeding Progenies Of Spore In Pleurotus Ferulae Lenzi

Pleurotus ferulae Lenzi which is famous for its tasty flavor, unique flavor and thehigherfood for medicinal value, is one kind of marketprospect broad rare and preciousedible fungus. Study on the Pleurotus ferulae Lenzi breeding is very few, it is important toattain Pleurotus ferulae Lenzi strains with properties of good quality, high-yield andvigorous adaptability through mutation. In this experiment, with the foundation of results,5 Pleurotus ferulae Lenzi parent strains and 129 crossing progenies new strains were usedas testing material on the basis of studing on mutation and hybrid. A series of selectingexperiment were explemented in order to attain Pleurotus ferulae Lenzi strains withproperties of good quality.Fifty-eight new cross combinations were obtained by Microscope checking andantagonist response.When mutually complementary enzyme bands and new enzyme bands of thecombinations appeared twenty-nine crossbreeding filial generation by comparison new ofenzyme bands between parent strains and cross combinations, the heterosis of thecrossbreeding filial generation was stronger.Through mycelial colour, mycelial growth, mycelial density and high temperaturetolerance compared experiment, WY Pleurotus ferulae Lenzi 4×26,YY11×32,YY11×29,WY Pleurotus ferulae Lenzi 10×4,WY Pleurotus ferulae Lenzi 7×29,WY Pleurotusferulae Lenzi 6×12etc have strong growing trend, big density and they grow quickly. WYPleurotus ferulae Lenzi 7×2,YY12×31,W Pleurotus ferulae Lenzi 4×26,YY1×33 havethe ability of high temperature tolerance. Strains WY Pleurotus ferulae Lenzi 4×26,YY11×32,WY Pleurotus ferulae Lenzi 6×31,YY3×22,WY Pleurotus ferulae Lenzi 6×12,YY11×29,WY Pleurotus ferulae Lenzi 10×4,W Pleurotus ferulae Lenzi 10×29,WYPleurotus ferulae Lenzi 4×32 and WY Pleurotus ferulae Lenzi 7×29 have the ability of lowtemperature tolerance. Activity of Carboxymethylcellulose, Lactase, Amylase, Laccase,Catechol oxiase and Polyphenol oxidasea of strains were different. The results showed thatthere is significant Positive correlation between average growth rate of culture and activityof Carboxymethylcellulose, other enzyme activity show some negative but correlation is not significant.Compared about he mycelium growth, grow Periodicity and the biological efficiency,The strain in most still had the growth speed quick superiority takethe cotton seed hullsubstrates on the main matrix culture medium. The variance analysis indicated significantcorrelation between growth rate of mother cultures and growth rate of culture spawn. In thesame Cultivation management condition, strains have different cmpleted bagperiod, primordium stage and high flush stage. W Pleurotus ferulae Lenzi 4×26,WPleurotus ferulae Lenzi 7×2,W Pleurotus ferulae Lenzi 6×12,YY11×29,W Pleurotusferulae Lenzi 10×4,Pleurotus ferulae Lenzi 10×29和W Pleurotus ferulae Lenzi 7×29 areearly-mature strains. Some of cross combinations such as WY Pleurotus ferulae Lenzi10×29,WY Pleurotus ferulae Lenzi 7×29,YY7×26,YY11×29 and YY11×31, showstronger heterosis in fruitbody yields, and their biological efficiency are higher than parentstrains, they show heterosis in the yield characteristics.Different Pleurotus ferulae Lenzi strains are different in pileus diameter, pileusthickness, stipe diameter, stipe length and weight of fruit. Generally speaking, YY10×32,YY7×26,YY2×3,YY11×29,YY13×26,YY2×8,WYPleurotus ferulae Lenzi 7×29and WY Pleurotus ferulae Lenzi10×29 are much better than others, which show biggerPileus, shorter Stipe, higher yield of fruit. Pleurotus ferulae Lenzi parent have muchNutrition than cross combinations. From analyzes to food high protein content requestangle, the nutritional value of YY10×32, YY7×26,Pleurotus ferulae Lenzi 7×12 are higher.