Study On Cryobiological Of Porphya Haitanensis In Cold Preservation

Larva of strain 1 and strain 2 of the algae Porphya haitanensis were preservation for experiment. Effects on different sizes, water ratio and temperature of cryobiological of P. haitanensis in cold preservation were investigated. Effects on cryobiological of P. haitanensis under cryoprotectant in cold preservation were studied. Application and quality analyses of refrigerated nets of P. haitanensis were studied too. The results showed:1. Based on survival and growth of P. haitanensis, there are the optimum water ratio that is 10%～30%, 10%～15% had best. Thalluses( algae sizes were 1, 3, 5, 7, 9cm)were preservatized in -20℃for 30days.Thalluses of 1 cm and 3 cm groups grew rapidly and maturated slowly than other dealing groups in the same culture times.2. In normal stored temperature groups, water ratio of thalluses had little influence on survival. At groups of 17, 20, 23, 26, 29℃, the lower of normal stored temperature, the better of store efficiency. At 17℃for 10 days, a few cells of P. haitanensis were dead. It was found that along with cold preservation temperature reducing, the survival rate of P. haitanensis were raising in 5～-25℃preservation. After 30d cold preservation in -20℃and -25℃, the growth of length and weight of P. haitanensis were 20%～40% higher than other dealing groups. Recuperation of P. haitanensis relates to temperature of water. In 20℃cold preservation, the survival rate was 100%. After 10 days'and 30days'cold preservation the survival rate of P. haitanensis (water ratio was 10%～15%) both were 100%; after 720 days'cold preservation the survival rate of P. haitanensis was 78.0%. In addition, the survival rates of P. haitanensis were reducing in along with cold preservation time.3. In normal temperature, there was lower cell survival of P. haitanensis in different concentrations of Glycerin and DMSO than in different concentrations of Glucose and Sucrose. Preservatized in Glycerin, it had higher survival rate than in the same concentration of DMSO. Preservatized in Glycerin for 30 days, the survival rate was 100%. Preservatized in Glucose and Sucrose for 15 days, the survival rate was 0%. Orthogonal experimental results showed: Preservatized in combination of 20% Glycerin plus 5% DMSO for 15 days, the survival rate were 100%. Different combination of DMSO, Glycerin and Glucose showed relatively good preservation effect. After 90 days preservation, the cell survival rate obtained 95%.Salinity and thickness of cell wall had some bearing on preservation effect. When salinity was 25, the cell viability was comparably higher than in other salinity. The thickness of cell wall had some impact on the storage effect. In the same strain, the cells with thick cell wall possessed stronger low-temperature resistance and higher survival rate than those with thin cell wall preservatized for 30days. In the different part of the same plant, some survival rate differences also exist after preservation for 30 days. It was found that the better of preservation, the higher of total volume of phycobiliprotein of P. haitanensis; survival and growth of P. haitanensis were related to volume of phycobiliprotein.4. There were significant difference from desiccation between P. haitanensis thallus and unwanted-alga (Enteromorpha intestinalis, Enteromorpha clathrata, Ulva pertusa, Gracilaria asiatica and Ectocarpus confervoides). Large scale unwanted-alga were dead after 2 hours'desiccation; some of cells of P. haitanensis had been dying after 3 h hours'desiccation (water ratio were lower than 10%). Large scale unwanted-alga were dead after 5 days'cold preservation; the survival rate of P. haitanensis (water ratio were about 70%) were higher than 90%. Large scale unwanted-alga were dead after 0.5 hours'desiccation and 5 days'cold preservation; the survival rate of P. haitanensis (water ratio were 10%～15%) were 100%Benthic diatom (Cocconeis spp.and Navicula spp.) had some adaptability for desiccation and cold preservation, lower than P. haitanensis, and higher than large scale unwanted-alga.5. In algal culture, the outputs of P. haitanensis dealing for cold preservation were higher than those had without dealing. Moreover, alonging with harvest times increasing, the volume of phycobiliprotein and chlorophyll of P. haitanensis were also decreasing.