Effect Of Different Nitrogen Source And Energy Level On Performance And Reproduction Parameter Of Lacting Buffalo

This experiments were conduted to study the effects of different protein source and energy level on milk performance, biochemical parameters of blood, endocrine hormone, and reproductive performance, to find the fators of effecting performance of buffalo and select best diet.During this experiment, 24 buffaloes were used, they were divided into 6 teams, each team had 4 buffaloes, they were feeded 6 kinds of dietary treatment, the 2*3 factorial trial involves two energy level (6.61 MJ/kg and 7.05 MJ/kg) and 3 nitrogen source, mainly study the effects of different nitrogen source and energy level on performance of buffalo.The results of this experiment showed: 1. feeding high energy level diet, weight gain increased significantly (P<0.05) , DM intake also increased, but not significantly; milk yield increased significantly (P <0.05), fat percentage feeding high energy level increased and low energy level team decreased ,but not significantly, other milk composition had not significant effects; feeding high energy level diet, energy efficiency was significant higher than low energy level team (P<0.05) , protein efficiency had the same result; there were no significant difference in all nutrient digestibility except crude fiber, high energy level team was significant higher than low energy level team; the concentration of triglyceride and glucose in high energy level team was lower than in low energy team, but not significantly, the concentration of galactin in high energy level team was significant higher than in low energy team (P<0.05) , the concentration of insulin had the same conclusion; the concentration of reproductive hormones in high energy level team was higher than in low energy team, but not significantly; the days of estrus and hybridization in high energy level team were fewer than in low energy team (P <0.05) .2. feeding the diet adding to feather meal, buffalo's DM intak increased, but less thanother treatments, but not significantly; milk yield was significant more than other treatments(P<0.01) ,protein and SNF percent in milk which add to phosphate urea were highest, significant higher than adding to feather meal (P<0.05) , lactose percent also higher than other two treatments, but not significantly; energy efficiency adding to feather meal was higher than other two treatments, but not significantly, protein efficiency adding to phosphate urea was highest, the control group was lowest, but not significantly; the digestibility of fiber and fat in phosphate urea team were significant higher than feather meal team (P <0.05), and the control group was significant higher than feather meal team (P <0.05) ; the concentration of total serum protein and BUN in control group was higher than in feather meal group and phosphate urea group, but not significantly; the concentration of T4 in feather meal group buffalo was significant higher than phosphate urea group and control group; the concentration of Follicle stimulating hormone was higher than other two groups, but not significantly; the days of estrus and hybridization in feather meal group buffalo was shorter than control group, and phosphate urea group was longer than control group, but not significantly.