Research On Pfaffia Paniculata Qucik-Propagtion In Vitro Culture

The research on Pfaffia paniculata quick-propagation in vitro culture was done to solve the shortage of Pfaffia paniculata seed source for introduction and cultivation. The stem apex, tender leaf, stem of Pfaffia paniculata were used as explants. The experiments are including shoot induction and multiplication, callus induction and differentiation, roots differentiation, rooting plantlets transplantation and etc. The results are shown as below:1 The culture of stem apex inductionThe stem apex of Pfaffia paniculata were used as explants and cultivated in the basic medium MS, N₆, B₅, White supplied with different concentration of hormone. The result shows that the cluster buds were obtained after forty-day induction culture in the medium of MS+BA1.5mg/L+NAA0.5mg/L, and the rate of shoot multiplication was 3.22.2 The culture of shoot multiplicationThe cluster buds were cut into a single bud and cultivated in the sub-enrichment culture medium MS adding different concentration of 6-BA, KT, ZT and NAA. The best medium is MS supplemented with concentration of 6-BA 1.2mg/L and NAA0.2 mg/L. The rate of shoot multiplication was 3.92 and the buds grow healthy and strong.3 The culture of callus inductionCallus can be inducted by tender leaf and stem, but the callus inducted by tender leaf is better than that of stem. MS+2, 4-Dlmg/L+20% CW medium is suitable for callus induction.4 The culture of rooting1/2MS medium supplemented with proper concentration of NAA, IBA or PP₃₃₃ caninduce rooting. When rooting induced by PP₃₃₃, plantlets are short and strong, the roots are burly, and the numbers of roots are less. PP₃₃₃ can prove the survival rate of transplantation. 1/2MS+NAA1.0mg/L and 1/2MS+PP₃₃₃0.2mg/L medium are suitable for inducing roots.5 Plantlets transplantationThe plantlet was took out, washed the medium of plantlet's roots and cultivated in sand, vermiculite or soil, the result is that the survival rate cultivated in sand is higher than any other medium. The survival rate of rooting plantlets treated by PP₃₃₃ could be 90%.6 The analysis of chemistry compositionAfter plantlets grown for one year, the comparative analysis was done by major chemistry composition, total mogroside content, amino acids and trace elements. The saponin constituents of culture with twelve month Pfaffia paniculata and provenance were same. Total mogroside content is 6.54%, slightly less than original Pfaffia paniculata 7.32%; the both plantlets in vitro culture and original Pfaffia paniculata had the same kinds of amino acids and trace elements, and the content is 2.6%, original Pfaffia paniculata is 2.2%, slightly more than original.