| Magnaporthe grisea is an important ascomycete that causes rice blast. Studying of the disease-caused basis of M. grisea is not only beneficial for rice blast control, but also serve as a model for revealing other fungal pathogen-plant interactions.Autophagy is a ubiquitous process that degrades and recycles long-lived proteins and organelles under starvation in all eukaryotic cells. So autophagy has been presumed to be involved in cellular architectural changes that occur during differentiation and development, presumably via its role in organelle and protein turnover.In this study, MgATG3, MgATG4 and MgATG7 genes from M. grisea were cloned. The role of MgATG4 in pathogenicity was analyzed by targeted gene replacement. The results are showed as follows:1. Using the sequence of ATG3,ATG4 and ATG7 genes in yeast , we found the homologous sequence from M. grisea genome databases, named as MgATG3,MgATG4 and MgATG7.2. MgATG3,MgATG4 and MgATG7 genes replacement vector were constructed, and hygromycin-resistance transformants were obtained by MgATG4 gene fungal transformation. Knockout mutants were identified by PCR and Southern blot. Single copy was detected by genomic Southern blot in the genome of Guy11 for MgATG4 gene.3. Compared to Guy11, the colony modality ofΔMgATG4 was much different. The growth rate of the mutants on CM, CM-C, CM-N and CM with 1M NaCl had no obvious change.4. Conidia germination and appressoria formation of mutantΔMgATG4 was suppressed, compared to wild type. The pathogenicity ofΔMgATG4 mutant was partially reduced on barley.5. MgATG4 eGFP fusion expressing vectors, named as PBNEATG4, is constructing.
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