The Effects Of Different Dietary Energy Levels On Ovarian And Uterine Expression Of IGF-1 Receptor And EGF Receptor MRNA In Prepubertal Gilts

Firstly, this paper reviews"Advances in study of the relationship between IGF-1R,EGFR and reproductive system"and"The effects of energy on reproductive ability". In the part of experiment, nine litter-mate JunMu No.1 (bred by Quartermaster University of PLA in 1999) gilts were used in the study, which were randomly allocated to three groups (n=3) and fed with a high level digestive energy (Group H), a low level digestive energy (Group L), and a control group digestive energy (Group M) diet, respectively. To reduce the diversity of growth performance, nutritional metabolic status, and the adaptability to the new environment, gilts were accommodated in an acclimatizing period for 7 days before undergoing feed energy experiment. Ovaries and uteruses were collected, after 2 weeks energy treatment, and processed for determination of the absolute quantities of IGF-1R and EGFR mRNA by using real-time PCR. The ovaries and uteruses in the Group H expressed significantly greater amount of IGF-1R and EGFR mRNA than in the Group M and Group L (p<0.05). But only the ovaries in the Group L expressed significantly less amount of IGF-1R and EGFR mRNA(p<0.05) than in the Group M. The present study demonstrated that high energy intake markedly enhances the ovarian and uterine expression of IGF-1R and EGFR in prepubertal gilts, whereas, insufficient energy intake markedly inhibits such expressions. The result suggests that IGF-1R and EGFR might be involved in mediating the effect of energy intake on puberty.