The Ultrastructural And Histochemical Study Of Anther Development In Lycium Barbarurn L.

The ultrustructural featers of anther of Lycium barbarurn L. was studied using transmission electron microscope technique during microsporogenesis and the development of male gametophyte. The characteristic of distribution of nutriment during the development of anther was also studied using cytochemistry technique. The results as following:1. The characteristic of cytoplasm changeDuring the microsporogenesis and the development of male gametophyte some organelles displaied quantitative and structural change which means cytoplasm taking place"cytoplasmic reorganization": from the prophase I to the telophaseâ…¡of meiosis, ribosomes decreased in number and the structure of mitochondria became reduced. It shows that the cytoplasm has happened dedifferentiation. After the meiosis the number of ribosomes increased and the inner structure of mitochondria became complex, suggestting a redifferentiation process of cytoplasm. At the vacuolation stage of the microspore, ribosomes and mitochondria of microspore toke place decreased in number again, and plastids became swell in shape reflacting a dedifferentiation process of microspore, and then resumed again at the early of bicellular pollens suggesting a redifferentiate process. It means that there were the"cytoplasm reorganization"phenomena in both the microsporogenesis and the development of male gametophyte, but the purpose of both"cytoplasm reorganizations"between the microsporegenesis and the development of microspore was different. Tthe former"cytoplasm reorganization"was to eliminate the sporophytic communicating material preparing microspore development, and the later"cytoplasm reorganization"was to eliminate the microspore's communicating material preparing male gametophytic development.