Experimentation And Study On Immunization Of Inhibin Gene Vaccine PCISI In Nanyang Cattle

Techniques such as molecular clone, gene immunization, enzyme-linked immunoassay (ELISA) and radio immunoassay (RIA) etc were applied to study the immune response to inhibin gene immunization, effects on the reproduction and reproductive endocrinology in cattle. The different immune effects caused by different purity of vaccine, dose and adjuvant were studied to search for the best way to increase the reproduction of cattle, reduce the costs and popularize the new technology. The main content presents as follows:1. The study of immunoreactive character of inhibin pCISI gene immunization on Nanyang cattleIn order to analyze the immunoreactive character of cattle to inhibin gene immunization and its affecting factors, the different dosages, purity of inhibin fusion expressing plasmid pCISI were used to immune the cattle twice. The results showed that there were some sheep with positive antibody against inhibin on 10 days after primary immunization, and the average antibody P/N value of immunization group were higher than the control remarkably in the period of primary immunization (P<0.05), those in the period of booster immunization were greatly remarkable higher than the control (P<0.01). After booster immunization the rate of cattle with positive antibody is from 16.83% to 52.75 % 46.7%, which was remarkably different. Both the level of antibody against inhibin and the rate of positive antibody, there was no remarkable difference in the four groups with different doses (0.75 mg, 1.5mg, 2.25mg, and 3.0 mg). The level of antibody against inhibin of 3.0mg immunization group was the highest (1.97±0.62), and the rate of positive antibody of 2.25mg immunization group was the highest (56.62%, 13/23). The immunization effect of highly purified pCISI (OD₂₆₀/ OD₂₈₀ between 1.8 and 2.0) was slightly better than pCISI with lower purity (OD₂₆₀/ OD₂₈₀ between1.7 and 1.8 or between 2.0 and 2.1), but the difference wasn't remarkable (P>0.05). Combined the dose with the purity, the effect of immunization response of 0.75mg inhibin fusion expressing plasmid pCISI in groups with lower purity was the best, and the effect of 2.25mg pCISI in groups with high purity was...