| The self-incompatibility genotypes (S-genotypes) information will be useful for the selection of pollinizers and designing of crosses in cultivar improvement program of Japanese pear. However, S-genotypes identification of Japanese pears originated in China have been seldom reported. This paper reports the self-incompatibility genotypes of 23 Japanese pears identified by PCR-RFLP. Furthermore, 2 new S-alleles were discovered, for which the labels S38 and S39 were proposed.1. Genomic DNA was extracted from young leaves by a CTAB method. The OD260/OD280 of most Japanese pears is between 1.7 and 1.9. The average yield of DNA ranged above 200 μg/g of sample. These results suggest that the purity of DNA extracted is suitable for molecular biological experiment.2. For 5 Japanese pears from Japan, 'Imamuraaki' (S1S6), 'Kikusui' (S2S4), 'Housui' (S3S5), 'Okusankichi' (S5S7) and 'Shinkou' (S4S9), the S-genotypes of the expected were identification.3. The S-genotypes of 14 Japanese pears originated in China and 4 originated in Japan were identified by S-allele-specific PCR-RFLP. They were as follows: 'Jinshui 1' (S7Sx), 'Jinshui 2' (S3Sy), 'Enoshima' (S5S8), 'Huali 1' (S1S3), 'Shounan' (S1S3), 'Cuiguan' (S1S4S5), 'Hangqing' (S3S7), 'Xizilu' (S1S4), 'Xinhang' (S1S3), 'Qingxiang' (S3S7), 'Sanhua' (S7Sz), 'Huangmi (Imamuranatsu)' (S1S6), 'Huanghua' (S1S2), 'Ninomiyahaku' (S3SW), 'Huali 2' (S3S4), 'Baozhuli' (S7Sa), 'Cangxixueli' (S5Sb), 'Guanyangxueli' (S7SC).4. The S-genotypes of Japanese pears originated in China were validated by the information of parent S-genotypes. All of the S-genotypes in 9 hybrid cultivars from China accorded with the expected S-genotypes in the progenies between their parents respectively except 'Huali 1'. 'Huali 1' ('Shounan' × 'Enoshima'), had the same S-genotype S1S3 as its maternal parent 'Shounan', but this was in contravention of the theory of GSI. The result of pollination tests between 'Shounan' and 'Huali 1' indicated that they had the same S-genotype. |
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