Isolation Of Microsatellite Markers From Porphyra Haitanensis And Their Application In Genetic Diversity Of Porphyra

Porphyra haitanensis is seaweeds of high nutiitive value, and it has a long history of culture in Fujian province.In this thesis, we first constructed a small-size genomic library of P.haitanensis and got 1500 positive recombinants.Among which we obtained 4 microsatellite sequences from 215 positive clones.Choosing 2 of the microsatellite loci in P.haitanensis and 3 microsatellite loci of Gracilariopsis(AJ512812,AJ512806 and AJ512804) from GenBank as primers.9 DNA samples(LYG,RD,CS,GL1,DXY,BBD,JJS,NHå’ŒLSD)of Porphyra were amplified,and a totle 31 DNA bands were detected.AJ512812 amplified 12 DNA bands were the most while AJ512804 only amplified 1 DNA bands, which was the least of them. Among 31 DNA bands, 83.9% were polymorphic. The data was analyzed by means of Nei's similarity coefficient, and a phenogram was constructed by using between groups linkage method. The result showed that the similarity coefficient of the 7 P. haitanensis from 58.% to 96.8%.The highest similarity coefficient is 96.8% which among 3 wide P.haitanensis.The results indicated a high genetic varitions of P.haitanensis,and make a genetic foundation for excellent breed select.According to the dendrogram we can find that the 9 Porphyra were divided into two teams. The 7 P.haitanensis samples had closely relationship in the same team and the 2 P.yezoensis had closely relationship. However Nei's genetic distance between CS and GL1 was larger than that between P.haitanensis and P.yezoensis.It indicated that different geographical localities where the sample were...