The Study On Immunoregulation Of Fusion Gene Of Porcine Interleukin-6 And Interleukin-4 In Animals

In order to develop safe and effective immune adjuvant of animals, we reconstructed the porcine IL-6 and IL-4 gene, and get the novel fusion IL-46 gene. Furthermore, we get VRPIL46 VRPIL4 and VRPIL6. The Chitosan nanoparticle wasprepared by ion-crossing method, and this VRPIL46 was entrapped with chitosannanoparticles (GNP) and utilized to immunize Kunming mice which in two ways at the age of 21 days. The peripheral blood was weekly collected from the tail vein of inoculated mice to detect the effects of VRPIL46 Chitosan-nanoparticles on immune responses of mice. The results were founded that, compared with those of the control, the content of immunoglobulins, dominantly IgG, including IgA and IgM, and specific antibodies to the vaccines against Swine fever, the Pasteurellosis and streptococcus mounted significantly in the sera from the VPIL46-CNP, vaccinated mice, the amount of IL2, IL4 and IL6 increased remarkably in the sera of immunized mice, so were the number of white blood cells, lymphocytes and monocytes, granuloytes of the mice immunized with VPIL46-CNP. After the challenge by virulent Pasteurellosis and streptococcus, these immune values were respectively elevated to different extent in the mice vaccinated with VPIL46-CNP. The most of immunized mice survived, while the control mice fell ill with the evident lesions with diffuse hemorrhage in stomach, small intestines and peritoneum.