Studies On Tissue Culture And Gene Transformation Of Eucalyptus Urophylla×Grandis

In this paper, we studied systematically tissue culture of Clone 3226/3228 of Eucalyptus urophylla×grandis. The system of tissue culture regeneration system and agrobacterium-mediated transformation of Eucalyptus urophylla×grandis was established and some Kan-resistant plantlets of Eucalyptus urophylla×grandis had been obtained successfully. The results were included in the following:1. The best regeneration medium is A+6-BA_{0.5～1.0mg/L}+NAA_{0.1～0.2mg/L}.The aseptic seedling can keep the strong growth potential as well as regeneration in high multiplication times, and have few vitrified shoots.2. The lower light intensity is fit for regeneration and multiplication of the two clones of Eucalyptus urophylla×grandis.3. 25 days' generation is favorable for the regeneration and multiplication. The aseptic seedling can regenerate in high multiplication times as well as keep the strong growth potential.4. Active carbon can make the browning aseptic seedling and vitrified shoots grow normally, but have no effect on multiplication.5. A+6-BA_0.1mg/L+NAA_0.2mg/L is the best seedling-strong medium, it can promote the stem lignification and enlarge the number of effective leaves.6. IBA can promote the two clones of Eucalyptus urophylla×grandis root induction obviously, and the best concentration is 1.0～1.5mg/L. IAA and NAA can promote the root induction too, but have no obviously effect. The high concentration of auxins will inhibit the growth of root.7. In the same auxins concentration, half inorganic salt of A medium is better than total inorganic salt to the two clones of Eucalyptus urophylla ×grandis root induction.8. The leaf-fragment and shoot of two clones of Eucalyptus...