Fertilization In Vitro And Embryo Transplanting Of Quail

The present study was conducted to solve the problem that the complex structure of poultry eggs and special process of embryos' development for direct practice the project of gametes and embryos in quail even in poultry, and to promote the process of transgenic bird embryos frozen nuclear transferring and sex manipulation. Developing the advantage of poultry as bioreactor, to get special biologic production and to make the poultry biotechnology a furthermore development. Some aspects were researched as following:1 In vitro culture of quail embryosFour different methods were used to culture the quail embryos in vitro, each methods includes 100 examples, the hatchability of method I II III IV was 0 ,30% , 10% ,20%. Compared with the contrast troupe, the difference of the results was significant (p<0. 05)and the effect of method Hand IV was the best. From the record of embryos' survival rate in every stage and the comparison of hatchability, the survival rate of quail cultured by method I and III was similar , the survival rate between method II and IV was similar .2 In vitro fertilization and culture of quail100 female quails about six months old which were separated from the male quail were choosen, after a long term observing in egg discipline .quails were killed at 16:30 and the ovum were collected from abdominal cavity or genital tract , then were absolutely cultured in vitro after fertilization in vitro .The development of the embryo were observed by different collected part, the result showing that: only the ovum collected in infundibulum can integrate with the sperm . the fertility rate was 32% , embryo development rate was 20% after 24 hours .The infertile egg with shell which was produced from the female quail was directly injected sperm by microinjection , the fertility rate was 30%...