Evaluation On Security And Toxicity Effect Of Diniconazole On Main Tomato And Capsicum Pathogens

In this paper, the influence of diniconazole on tomato and capsicum growth was studied. Diniconazole was used to soak tomato and capsicum seeds, to spray leaves at seedling and adult stage. Plant height and the content of the lignin, chlorophyl at seedling stage and the content of the lignin, chlorophyl at adult stage were determined. The impact of diniconazole on secondary xylem of the tomato and capsicum at adult stage was observed. In the lab, the control effect of diniconazole mixture on tomato Botrytis cinerea and capiscum Colletotrichum capsici was determined. Diniconazole, as the study object, was sprayed in order to proceed the study of residual degradation dynamics. Residue was detected by GC-MS.Primary findings as follows:1. Diniconazole was not suitable for seed soaking,the rate of capsicum in bud would be severely restrained on dosage at 30μg/mL of the diniconazole;as tomato seed soaking dosage,it was inadvisable well above 60μg/mL. At seedling stage of tomato and capsicum, diniconazole dosage should not be over 30μg/mL;otherwise, the growth of tomato and capsicum would be severely restrained and severe stunt,shrinkage of leaf surface and stalk thickness were caused in upper time.2. When the dosage of diniconazole was over 30μg/mL, the leaf color of capsicum would be changed into thick green at seedling stage;the result of determination indicated that the content of the chlorophyll was increased by the dosage and increase so much as concentration of the dosage. The content of the chlorophyll was also increased at adult stage with the dosage on capsicum,but it was not evident to influent on leaf color and there was no shrinkage of leaf surface. When the concentration of the dosage to tomato at seedling stage was over 30μg/mL, the content of the chlorophyll was increased;but the color of leaf was not changed and the content of the chlorophyll was increased at adult stage.3. In three weeks when tomato and capsicum were transplanted, after the dosage was sprayed on the surface of leaves, the increase of Lignin content in stems and concentration of the dosage would be caused.4. It was not evident to influent on the secondary xylem tracheid cell of stems of tomato and capsicum by spraying diniconazole. When the concentration of the dosage was over 100μg/mL, remarkable augment of the diameter , shrinkage of the length of tracheid cell and the decrease of the number of tracheid cell on some cross section would be caused. It was small of diniconazole to shrink the length of the secondary xylem tracheid cell of stems,but it was big to shrink the diameter of cell,which was in some sort different with the size of tracheid cell by diniconazole.5. The determination of grey mold mushroom poison force mensuration on the binary dosage of diniconazole and baristin to tomato: when the rate of diniconazolet and bavistin was 10:1, the synergism coefficient SR was 1.52 greater than 1.5,which was represent to increasing effect, the rest rate was represent to additive effect. The determination of grey mold mushroom poison force mensuration on the binary dosage of diniconazole and arasan to tomato: when the rate of diniconazolet and arasan bavistin was 5:1, the synergism coefficient SR was 1.72 evidently greater than 1.5, which was represent to increase effect.6. The normal recommend medicament dosage of the 12.5% diniconazole wp was 3000 times. The result of this experiment showed that the original sediment of diniconazole at capsicum was 0.590mg/kg in 2h after spraying 1500 times'dosage once, and the diniconazole residue in capsicum was degradated by 44.71% after 3d, the rate of diniconazole clearing up at capsicum was up to 98.96% after 45d. Thus the residual degradation curve equation was C=0.3715e0.0574t, which was coincident to the formula C=C0·e-kt. The correlation coefficient was R = 0.9614 and the residue half life was T1/2=-ln2/k=12.08d.The correlation of the result was better.The speed of diniconazole residue in capsicum was high, thereof long-term residual could not be caused.The original sediment of diniconazole at tomato was 0.599mg / kg in 2h after 1500 times'dosage was sprayed once,and the diniconazole residue in capsicum was degradated by 39.52 % after 3d, the rate of diniconazole clearing up at tomato capsicum was up to 92.65% after 45d. After statistical analysis the residual degradation curve equation C=0.365e-0.056t was gained, which was coincident to the formula C=C0·e-kt. The correlation coefficient was R=0.9082 and the residue half life was T1/2=-ln2/k=12.38d.The correlation of the result was better.The speed of diniconazole residue in capsicum was high, thereof it could not cause long-term residual.Therefore,the extension rate of 12.5% diniconazole wp at tomato and capsicum 3000 times as recommend,and it is safe to plant nuisance free fruit tomato and capsicum.