Study On Reactive Oxygen Species Metabolism And Effect Of Signaling During Dormancy Process Of Prunus Persica

Peach (Prunus persica L.) is one of the most extensively cultivated temperate zone fruit tree and favorite fruit for people. But due to peach's sensitivity to environment, clear regional distribution of cultivating, short and concentrative mature period and soft and putrescible pulp, which lead to the difficulty for sale abroad. So the economic benefit is lower. In recent years, with the development of deciduous fruit tree protected cultivation, advancing or delaying cultivation has become the method of enhancing economic benefit. But one of the key issues is mastery the rule of dormancy. Experiment was conducted with several middle and late-maturing cultivars peaches which fitting for the area of Lanzhou, and researched the changes of endogenesis H2O2 content, O2.- and NO production rate and SOD, CAT, POD activity during dormancy stage and releasing dormancy. At same time, the research was studied on the changes of antioxidant enzyme and oxide after the treatment of extrinsic regulator and different concentrations of SNP and H2O2. In order to research ROS metabolism and which the effects of signal for dormancy released,which accumulate basic information for the mechanism of peach dormancy, and provide technology reference for fertility of peach protected cultivation. The main results as following:(1) With the gradually process of dormancy, O2.- and NO production rate and H2O2 content in the buds increased, but declined when dormancy released. The time variouty of NO production rate dropping sharply was earlier than O2.-, in succession the content of H2O2 dropped sharply, which suggested that NO play an important role of signal transduction in inducing dormancy release of the peach.(2) Content of H2O2 decreasing time was basically consistent with endodormancy release (Mid-maturing peach finishes nature dormancy in about middle December, Lat-maturing peach in about late December), which showed the increasing of ROS content was the significant characteristic of dormancy, the decreasing of it was the symbol signal of dormancy release, or a signal transduction material of dormancy release.(3) Treated by extrinsic regulator the results indicated that GA3(100mg/l) and l 6-BA (100 mg/l) could decreased NO and O2.- production rate and H2O2 content, but advanced the time of...