Study On Molecular Marker Of Leafy Triat Gene And Analysis Of Related Traits In Maize

Maize is one of the main crops,which plays an important role in the national economic development . For a long time,lacking of germplasm resources was the leading problem of the maize breeding in our country .The rapid development of livestock has a big demand on embodied germplasrns of forage maize,which was deficient especially,so it is necessary to add new germplasrm and expand use of germplasm resources.Leafy maize material is a new-type of germplasm derived from U.S.A,leafy maize had 8-25 leaves above the ear, better stalk strength , much taller plant height and more flourishing root than non-leafy maize,their stalk,vas stalwart,their resistance was strong synthetically . In order to uses well this idioplasm resources, it is necessary to study further heredity of leafy trait.F₂ population and two parents inbred lines Y53 with 4L and 14L above ear were used. The heredity rule of the leafy trait, relevance of the leafy trait and the growth duration and the molecular marker of leafy trait was investigated in the study. The results as follows:1. The heredity analysis was made about the leafy number above the ear by the F₂ population distribution. The result indicated that the leafy trait was the quantity character. The leafy trait was controlled by major genes and minor genes, it was not by the single gene, also was not together by the completely equivalent minor genes .2. The field investigation of the leafy number above the ear and the growth duration indicated: the correlation of the earing period and change of the leafy number above the ear, the wiredrawing time and change of the leafy number above the ear, disperse the powder time and change of the leafy number above the ear all were the extremely significant linear. R was 0.8465**, 0.8911**, 0.8595** respectively.3. Among 125 SSR primer pairs, 32 amplify polymorphism between two parents , distributed in 10 linkages were abserved the rate of polymorphism was 25.6%.Eleven SSR markers on the chromosomes 1,3,4,5,6,7 and 10 ,respectively were detected,explening 0.0545-27.56% of phenotypic variance.