Investigation On Molecular Epidemiology Of Type 2 Porcine Circovirus (PCV2)

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) that has become an emerging and important disease in swine. In the present study, suspicious samples collected from Jiangsu area were detected for PCV1/PCV2 by multiplex PCR, and 18 pigs with PCV2 infection were detected from the tissues of hearts, livers, spleens, lungs, kidneys, brains, groin lymph nodes, mesentery lymph nodes and tonsils respectively. PRRSV and PCV2 were detected in the Porcine Alveolar Macrophage (PAM) from pigs with respiratory diseases. Five PCV2 strains were isolated from the clinical samples. The complete genome sequences of these five isolates were determined and compared with those of other PCV2 strains.1,162 suspicious samples collected from Jiangsu area were detected for PCV1/PCV2 by multiplex PCR, and the results showed that 72 cases were PCV2-positive, 17 cases were PCV1-positive and among which 7 cases were positive for both PCV1 and PCV2. Most of samples collected in September, October and November during a year, and the positive rate was up to 54.67%. Samples were overwhelmingly collected from nursery and fatting pigs, and the positive rate of them was 26% and 68% respectively. The epidemiology investigation indicated that this disease seemed to become epidemic in Jiangsu regions.2,By using multiplex PCR, 18 pigs with PCV2 infection were detected from the tissues of hearts, livers, spleens, lungs, kidneys, brains, groin lymph nodes, mesentery lymph nodes and tonsils respectively. The results showed that among the tested 18 pigs, PCV2 were detected in all tissues of the 7 pigs; and the results also showed the positive rates of lungs , groin lymph nodes and tonsils were all up to 100﹪which indicated that the three tissues were the optimal ones for PCV2 detection.