Hydrolysis Production Of Small Peptide From Sheep Blood And Effect Of It On The Metabolism Of Nitrogen In Rumen

According to the new nutrition theory of peptide, this study initially added different small peptide into diet of sheep. Firstly, establish preparation method of obtaining small peptide from blood of sheep. Secondly, five male mature Merino sheep, fitted with permanent cannula in the rumen, T-type cannula at the proximal duodenum, were fed with the same basal diet. Three periods were designed, adding8% (blood meal / beneficiated burden material) different treatment blood meal (dry blood meal\ acidolysis blood meal\ enzymolysis blood meal) into basal diet in the different period in order to study effects of the different blood meal on digestion and metabolism in rumen.Experiment 1: The results showed that blood homogenated was the best substrate of reaction; the best enzymolysis conditions of sheep blood were: the type of enzyme was acidic proteinase; pH was 3.0; temperat- ure was 50℃; concentration of enzyme was 5%; concentration of substrate was 6%; reaction time was 4 hours. The best acidolysis conditions of sheep blood were: 30% industry hydroc- hloric acid; hydrochloric/ whole blood was 1/4; acidolysis temperature was 100℃; reaction time was 2 hours. Contents of small peptide in two kinds of product both were 60%.Experient 2 : The results showed that the intake in 3 groups were 1247.54±77.56,1231.93±55.52,202.49±8.43g/d·sheep respectively;the intake of crude protein were 219.96±14.00,221.64±10.00,1124.93±46.85 g/d·sheep respectively;pH of rumen were 5.80±0.17,6.05±0.38,5.82±0.29 respecti- vely;concentrationg of NH3-N in rumen were 18.21±5.07,16.46±5.86,23.88±13.24 mg/100ml respectively;concentration of small peptide in rumen were 12.28±1.79,16.00±1.66,12.82±1.15 mg/ml respectively;concentrationg of NH3-N in duodenum were 14.80±3.42,12.00±2.53,16.29±4.17 mg /100ml respective- ely;concentration of small peptide in duodenum were 11.94±0.94,14.04±1.34,12.56±0.88 mg/ml res- pectively;concentration of MCP in duodenum were 4.10±0.70,4.70±0.6,4.30±0.50mg/g respectively. Conclution drawed from this experiment were: the DM intake in acidoly- sis group was decreased significa- ntly (p<0.05), but there was no different between control group and enzymeolysis group; concentration of MCP in acidolysis group and enzymolysis group were increased to 14%,5% (p<0.05); no effect of enzymolys- is and acidolysis blood meal in diet on change rule and extent of NH3-N in rumen., hight concentration of NH3-N in acidolysis group no impacted absorption and utilization in rumen ; comulation of small peptide in rumen was increased in enzymolysis group.