Vitrification Study On Bovine Oocytes And Embryo

The study on bovine oocyte vitrification frozen were carried out from 5 aspects: the vitricationfreezen on different maturation time of bovine oocyte; the bovine oocyte vitrified by different vitrificationfluid and the same vitrification fluid with different concentration; the different carrier used in the bovineoocytevitrificationfrozen;theoocytestayatthesamevitrificationfluidwithdifferenttime;theoocytetakedifferent layer number of cumulus cell when it vitrified frozen. I also take study on the bovine embryovitrificationfrozenfrom2aspects:thevitrificationfrozenonthedifferentdeveloptimeofbovineembryo;thedifferentcarrierusedinthebovineembryovitrificationfrozen.Theconclusionare:(1)The companany on the impact of the vitrication frozen on different develop time of bovine oocytefindthat:theimpactofvitrificationfrozenonbovineoocytewhichafter22hoursinvitro maturecultureisbetter. The oocyte morphologically normal rates after vitrification frozen is 94.14%.The bovine oocytecleavagerateis41.80%andtheblastocystrateis15.23%.(2) The companany on the impact of the vitrication frozen on the oocyte stay at the same vitrificationfluid with different time find that:the impact of vitrification frozen on bovine oocyte which stay in thevitrification fluid for30second isbetter.Theoocyte morphologicallynormalrates aftervitrification frozenis93.53%.Thebovineoocytecleavagerateis41.18%andtheblastocystrateis14.70%.(3)Through the contrast the impact of the different carrier used in the bovine oocyte vitrificationfrozen found that: the impact of glass straw carrier and plastic open pulled straw carrier which used inbovineoocytevitrificationfrozenisbetter.Whenuseglassstrawcarriertheoocyte morphologicallynormalrates after vitrification frozen is 91.20%.The bovine oocyte cleavage rate is 41.30%and the blastocyst rateis14.52%.(4) Through the contrast the impact of vitrification frozen on oocyte which took the different numberof cumulus cell layer I found that: the impact of vitrification frozen on oocyte which take 2~3 layers ofcumulus cell is better than other number of cumulus cell layer. After vitrification frozen the oocytemorphologicallynormalrates is90.79%.The bovine oocyte cleavage rate is40.13%and the blastocyst rateis15.13%.(5) Through the contrast the impact of vitrification frozen on oocyte which vitrified by differentvitrificationfluidandthesamevitrificationfluidwithdifferentconcentrationfoundthat: thecombinationandconcentrationofEDFS30hasbetterimpactofbovineoocytevitrificationfrozen.(6) Through the contrast the impact of vitrification frozen on the different develop time of bovineembryo I found that: The impact of vitrification frozen on bovine blastocysts stage is better than theimpact of vitrification frozen on bovine morula stage. After vitrification frozen the bovine blastocystsmorphologically normal rates is 85.71%. After invitro culture the developing rates of bovine blastocystsis34.13%.(7)The impact of glass straw carrier and plastic open pulled straw carrier which used in bovineblastocysts vitrification frozen is better. But the impact of 0.25ml tubule carrier used in bovine blastocystsvitrification frozen is not prominence difference with them. So we can use the 0.25ml tubule carrier inbovineblastocystsvitrificationfrozen.