SRAP Molecular Identification Among Germplasms And Molecular Markers Related To Quality Of Carthamus Tinctorius L.

Safflower (Carthamus tinctorius L.) is the only cultivated species in the genus Carthamus L.(Compositae). It is a traditional Chinese medicinal plant widely applied in China. The tubular flower is usually used to treat gynaecological diseases, cardiovascular diseases, cerebrovascular diseases and so on.Due to the long natural and artificial selection, obvious differentiation have occured in safflower genus and rich germ plasm resources have been formed. There are some differences in safflower varieties from exterior appearance to inner chemical constituent and content. All these provide genetic foundation for selecting breeding and more choices for exploituring therapeuticc value of safflower. However, which inevitablely causes difficulties in the evaluation of germplasm resources. In this research, SRAP is used to analyze the genetic diversity of 25 safflowers including wild variety and identifyed the germplasm of safflower from molecule level. At the same time, the marker related to content of effective constituent ( hydroxysafflor yellow A ) correlation in safflower were detected by SRAP.1. The optimized application system was carriied out to analyze genetic variation of 25 varieties using 30 primer combinations. All varieties could be uniquely distinguished by 9 selected primer combinations. 483 bands, including 274 polymorphic bands, were detected by 9 primer combinations. The average for per-primer combination generated 53 bands, including 30 polymorphic bands, the polymorphic ratio is 57%. Uniweighed pair-group method of with arithmetical averages (UPGMA) cluster analysis was used to construct a dendrogram, according to the trees the varieties could be clustered into two groups ( one group is wild safflower and the NO.4 variety, the other group is 23 varieties) at the level of GS=0.29 by the first classification, so we suggested that the NO.4 might be come from wild safflower. The varieties could be clustered into six groups at the level of GS=0.43 by the second classification, besides the NO.4 and the NO.13, the other varieties are clustered into 5 groups. There are low genetic similarity in 23 varieties, different primer combinations could be distinctly distinguished one safflower from others.2. In order to determine the nature of the amplified fragments using SRAP markers, the special fragments distinguishing the wild safflower from varieties, obtained with different primer combinations, were sequenced. The sequenced fragments were both monomorphic, appearing in wild safflowers, and polymorphic. Their accurate length of the five specific fragments (SPW237,SPW219,SPW371,SPW292,SPW206) were 237bp, 219bp, 371bp, 292bp, 206bp respectively. All these offer effective approach for molecular iditification of Carthamus tinctorius L. and Carthamus lanatus L..