Proteomic Approaches To The Study Of Differentially Expressed Proteins From Rice Leaves In Response To Bacterial Leaf Streak, Xanthomonas Oryzae Pv. Oryzicola

Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. Oryzicola, is widely distributed in tropical and subtropical area, and now emerging as an important pathogen of rice in southern China. Typically, BLS can cause grain weight losses range from 5-40%.The highly susceptible hybrid rice variety-9311 was used in all our experiments. Total proteins are extracted from leaves 12h, 48h, 5d, and 10d after rice BLS bacterium inoculation. We have used two dimensional electrophoresis (2-DE) and Mass spectrometry (MALDI-TOF/MS) to identify proteins that are differentially expressed in response of BLS infection. From 2-DE data, 164 resolved differentially expressed proteins were detected, 94 of those were induced, and 70 of those were repressed. Selected spots were subjected to tryptic digestion followed by identification using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Total 86 proteins were identified. We got the polygenetic tree of NBS-LRR type proteins and the conserved domains of NBS-LRR-type and RLK/Kinase-like proteins. Basing on the result of protein sequences analysis, we designed the PCR probes and got 8 genes from cDNA of rice, including 5 RLK/Kinase-like genes, 2 NBS-LRR type genes and 1 PR-10 gene. One of the NBS-LRR type genes, protein No. 13-3-4, showed highest homology to the other same type genes was 91%.The study can direct the further research about pathogen-plant interaction and defense responses in rice. At the meantime, we analyzed 2 important kinds of response proteins, got 8 sequence fragments of...