The Isolation And Culture Of Endometrial Epithelioglands In Goats And The Effects On PBMC Of Swine

The experiment established the isolated culture system of endometrial epithelioglands, approached the method of separating and purifying the endometrial glands in sex-maturity goats and lambs, moreover, co-culture with PBMC. And we detected the influence of glands to PBMC by MTT. Provide the experimental proof to the interaction between endocrine in different physiological period and immune system,the results as follow:上1. Carred out the experiment of digesting the endometrium and separating the gland cells, proved that when digesting 4 h by collagenaseⅠat 37℃, can get enough cells and be profit for the separation. The digestion was passed over the 200 sieve, purified the cells after gentle centrifugalization and natural sedimentation. Identified the cells we had by immunohistochemistry, the purity rate endometrial glands of is more than 95%.2. Compared the growth characters of the endometrial epithelioglands in sex-maturity and lambs, proved that in different period the growth of gland cells were distinguished and the gland cells in lamb were grew more faster.3. Established a new co-culture method, and co-culture the epithelioglands with PBMC of swine. The result showed that the method can satisfy the requirement of interaction between the two cells. Compared with other method, it is easy to operate.4. Proved that the epithelioglands and conditioned medium both can proliferate the PBMC of swine, and the gland cells could inhibited the proliferation of PBMC which was induced by PHA-P. It suggested that the endometrial epithelioglands could influence the activity of PBMC, and participate the regulation of uterus immune balance.The experiment approached the condition of isolated culture the endometrial glands in goats, the purity rate of cells we had gotten was more than 95%, and co-culture with PBMC of swine, indicated that the gland cells could affect the PBMC directly.