The Research On Soil Enzymatic Activities Affected By Monochlorobenze

The pesticides play very important role in improving the qualities and yield of agricultural products because they are widely used, good effectiveness and low price. But the wide application of organochlorine pesticides lead to pollute soil and water. They can lead serious environmental problems such as reducing the quality of agricultural products, resulting high contents of pesticide's remains in soil and reducing soil fertility. they can finally have a harm on health of people and animals.Soil enzymes are one of the important parts of soil. They can catalyze all biochemical reactions in soil, have remarkable affect on soil nutrimental substance recycling, energy transforming, monitoring soil pollution and remediation of soil or water. Pesticides have effect on soil enzymes after they entered the soil, it is important significance for us to study soil pesticide ecological toxicity by soil enzyme in theory and practice, for example reactional mechanism between soil enzyme and pesticide, give suggestions on remediation and monitoring to pollution.In rescent years, many scientists in different countries have studied the relationship between soil enzymes and pesticides, gotten some inconsistence results in soil enzymatic activity. It is seldom to report the enzymatic kinetic characteristics on this area. The urease, invertase and alkalinephosphatase are important hydrolyzable enzymes that can catalyze the recycling of C, N, and P. In this paper we systematically studied not only the influence of monochlorobenze on the activities of the three kinds of enzymes in Lou soil and Red soil, but also the mechanism between enzymes and the pesticides by enzymatic kinetics. We got the results as follows:1. Ethanol can remarkably increase the urease activity so that they have significant positive correlation with reaction time prolonging; the increasing degree of urease activity increases in decreasing trend. We can use model of parabola to regress their relationship. Ethanol can show the higher substrate affinity, and accelerate the breakdown the complex. Ethanol can remarkably inhibit soil invertase activity, the reactional mechanism is total competitive inhibition between ethanol and invertase, including no-competitive and competitive. With reaction time prolonging, ethanol can slightly inhibit soil invertase activity.